Omotuyi, O., Oghale, O., MacDonald, I. (2017). Regulation of Intestinal GLP-1 and GLUT2 genes underlie hypoglycemia in Desplatsia subericarpa (Bocq)-Fed Wistar Rats. Journal of Herbal Drugs
(An International Journal on Medicinal Herbs), 8(2), 79-86. doi: 10.18869/JHD.2017.79
olaposi Omotuyi; Ovuakporie-Uvo Oghale; Idu MacDonald. "Regulation of Intestinal GLP-1 and GLUT2 genes underlie hypoglycemia in Desplatsia subericarpa (Bocq)-Fed Wistar Rats". Journal of Herbal Drugs
(An International Journal on Medicinal Herbs), 8, 2, 2017, 79-86. doi: 10.18869/JHD.2017.79
Omotuyi, O., Oghale, O., MacDonald, I. (2017). 'Regulation of Intestinal GLP-1 and GLUT2 genes underlie hypoglycemia in Desplatsia subericarpa (Bocq)-Fed Wistar Rats', Journal of Herbal Drugs
(An International Journal on Medicinal Herbs), 8(2), pp. 79-86. doi: 10.18869/JHD.2017.79
Omotuyi, O., Oghale, O., MacDonald, I. Regulation of Intestinal GLP-1 and GLUT2 genes underlie hypoglycemia in Desplatsia subericarpa (Bocq)-Fed Wistar Rats. Journal of Herbal Drugs
(An International Journal on Medicinal Herbs), 2017; 8(2): 79-86. doi: 10.18869/JHD.2017.79
Regulation of Intestinal GLP-1 and GLUT2 genes underlie hypoglycemia in Desplatsia subericarpa (Bocq)-Fed Wistar Rats
1Center for Bio-Computing and Drug Development, Adekunle Ajasin University, Akungba-Akoko
2Department of Plant Biology and Biotechnology, University of Benin, Benin City, P.M.B. 1154. Edo State, Nigeria
Abstract
Background & Aim:Indigenous people of West Africa use the whole-leaf of Desplatsia subericarpa (Bocq) in anti-diabetic soup delicacy. This study was designed to validate the anti-diabetic claims and delineating possible mechanisms. Experimental:RT-PCR method was used to investigate regulation of intestinal glucose transporter 2 (GLUT2) and glucagon-like peptide-1 (GLP-1), and pancreatic insulin, L-type voltage-gated calcium channel genes. Insulin exocytosis was also monitored using ELISA method. The kidney sample was investigated for biomarkers of injury (kidney injury molecule-1 (KIM-1) and interleukin-1-β (IL-1β)). Results: GLP-1 up-regulation, GLUT2 down-regulation and increased insulin exocytosis but not increased insulin gene expression was observed in animals after a 3-day culinary exposure to D. Subericarpa leaves. This mechanism may explain hypoglycemia in streptozotocin-induced diabetes in animals in this study. KIM-1 and IL-1-β genes were marked up regulated in normal animals exposed (14-day) to D. Subericarpa. Recommended applications/industries:D. Subericarpa whole leaf contains phytochemicals principles with anti-diabetic potency but may be nephrotoxic. Therefore, for clinical use, selective fractionation of active components from the toxic components is desirable.
1Center for Bio-Computing and Drug Development, Adekunle Ajasin University, Akungba-Akoko
2Department of Plant Biology and Biotechnology, University of Benin, Benin City, P.M.B. 1154. Edo State, Nigeria
Abstract [Persian]
Background & Aim:Indigenous people of West Africa use the whole-leaf of Desplatsia subericarpa (Bocq) in anti-diabetic soup delicacy. This study was designed to validate the anti-diabetic claims and delineating possible mechanisms. Experimental:RT-PCR method was used to investigate regulation of intestinal glucose transporter 2 (GLUT2) and glucagon-like peptide-1 (GLP-1), and pancreatic insulin, L-type voltage-gated calcium channel genes. Insulin exocytosis was also monitored using ELISA method. The kidney sample was investigated for biomarkers of injury (kidney injury molecule-1 (KIM-1) and interleukin-1-β (IL-1β)). Results: GLP-1 up-regulation, GLUT2 down-regulation and increased insulin exocytosis but not increased insulin gene expression was observed in animals after a 3-day culinary exposure to D. Subericarpa leaves. This mechanism may explain hypoglycemia in streptozotocin-induced diabetes in animals in this study. KIM-1 and IL-1-β genes were marked up regulated in normal animals exposed (14-day) to D. Subericarpa. Recommended applications/industries:D. Subericarpa whole leaf contains phytochemicals principles with anti-diabetic potency but may be nephrotoxic. Therefore, for clinical use, selective fractionation of active components from the toxic components is desirable.
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