Phytochemical response of hyssop (Hyssopus officinalis L.) to foliar application of jasmonic acid

Document Type: Original article

Authors

1 Research Center for Medicinal Plants & Ethnoveterinary, Shahrekord Branch, I.A.U., Iran Medicinal Plants Program, University of Massachusetts, Amherst, USA

2 Department of Medicinal Plants, Researches Centre of Medicinal Plants & Ethno-veterinary, Shahrekord Branch, Islamic Azad ‎University, PO Box: ‎‏166, Shahrekord, Iran

3 Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan 84156 83111, Iran

Abstract

Background & Aim:Jasmonic acid (JA) is regarded as endogenous regulators that play important roles in regulating stress responses, plant growth and development.Effect of foliar application of JA on the essential oil and oil chemical components of hyssop (Hyssopus officinalis L.) were investigated. Experimental:  This study conducted in a CRD with three replications and in experimental greenhouse, I.A.U., Shahrekord Branch, Iran at 2012. Experimental treatments included (I) water foliar application (control), (II) water + aceton foliar application (as a solvent), (III-V) 50, 100 and 200 JA µL. The essential oils obtained by hydro-distillation were analyzed by Gas Chromatography-Mass Spectrometry. Results & Discussion: The results of GC-MS showed that the major components of the oil were cis-3-pinanone (10-39%), trans-3-pinanone (4-28%) and β-pinene (27-34%). The results of analysis of variance of the experiment showed that different levels of the foliar application of JA do have significant impacts on chemical constitutes in the essential oil.Finally, foliar application of 200 µL JA on some of secondary metabolite production in H. officinalis oil could be partially changed. Recommended applications/industries: Abbreviation: JA: Jasmonic Acid, MJ: Methyl Jasmonate, GC-MS: Gas Chromatography-Mass Spectrometry µL: Micro liter, M: Molar; ANOVA: Analysis of Variance, CRD: Completely Randomized Design.

Keywords


Article Title [Persian]

واکنش فیتوشیمیایی گیاه زوفا (Hyssopusofficinalis L) به محلول پاشی اسید جاسمونیک

Authors [Persian]

  • عبدالله قاسمی پیربلوطی 1
  • ابوالقاسم گرگیج 2
  • مهدی رحیم ملک 3
  • بهزاد حامدی 2
1 مرکز پژوهش های گیاهان دارویی، دانشگاه ازاد اسلامی واحد شهرکرد، ایران بخش تحقیقات گیاهان دارویی، دانشگاه ماساچوست، آمریکا
2 2. گروه گیاهان دارویی،دانشگاه آزاد اسلامی، واحد شهرکرد، شهرکرد، ایران
3 3. گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه صنعتی اصفهان، اصفهان، ایران
Abstract [Persian]

مقدمه و هدف: اسید جاسمونیک به عنوان یکی از تنظیم کننده های رشد بر رشد و نمو، تولید متابولیت های ثانویه و هم­جنین واکنش به تنش نقش مهمی دارد. در این پژوهش اثر محلول پاشی غلظت­های مختلف اسید جاسمونیک روی گونه زوفا (Hyssopusofficinalis L.)  مورد بررسی قرار گرفت. روش تحقیق: آزمایش به صورت گلدانی در سال زراعی 91-90 با تعداد پنج تیمار و سه تکرار در قالب طرح کاملاً تصادفی انجام شد. تیمارها شامل آب مقطر (شاهد)، آب مقطر و حلال هورمون (استون)، غلظت­های مختلف شامل 50، 100 و 200 میکرومول اسید جاسمونیک بودند. اعمال تیمار در مرحله قبل از گلدهی به صورت محلول پاشی روی گیاه مورد مطالعه انجام شد. نمونه های اندام هوایی با استفاده از دستگاه کلونجر به روش تقطیر با آب اسانس­گیری گردید. اسانس به دست آمده جهت شناسایی ترکیبات به دستگاه کروماتوگرافی جرمی متصل به طیف سنج جرمی (GC-MS) تزریق شد. نتایج و بحث: نتایج نشان داد که مهمترین ترکیبات موثره در اسانس اندام هوایی زوفا شامل سیس -3-پینانون (10 تا 39 درصد)، ترانس-3-پینانون (4 تا 28 درصد) و بتا پینن (27 تا 34 درصد) بودند. غلظت های مختلف اسید جاسمونیک بر میزان ترکیبات اصلی زوفا اثرات معنی­داری داشتند؛ به طوری­که غلظت­های مختلف به­کار­رفته سبب کاهش یا افزایش تعدادی از ترکیبات ثانویه شدند. اختلاف معنی­داری بین مقدار ترکیبات ثانویه مهم با کاربرد غلظت 200 میکرومول جاسمونیک اسید در مقایسه با سایر تیمارهای مورد مطالعه مشاهده شد. توصیه کاربردی/ صنعتی: در زراعت متابولیک اگر تولید یک متابولیت یا متابولیت های خاص مورد هدف باشند باید با مدیریت های مهم زراعی و فرآوری آن (روش های خشک کردن، اسانس گیری و غیره) به این هدف مهم یعنی افزایش میزان اسانس در قدم اول و میزان متابولیت (ها) در گام دوم دست یافت. با توجه به نتایج این مطالعه برخی متابولیت های ثانویه به خصوص ترکیبات فنولی مهم در اسانس آویشن دنایی به محلول پاشی اسید جاسمونیک تا حدودی واکنش نشان دادند. 

Keywords [Persian]

  • زوفا
  • متابولیت های ثانویه
  • اسید جاسمونیک
  • GC-MS
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