Isolation of borneol and bornyl acetate from Ferulago macrocarpa by microwave irradiation

Document Type: Original article

Authors

Department of Chemistry, Faculty of Science, Golestan University, PO. Box: 49138-15739, Gorgan, Iran;

Abstract

Background & Aim: A simple and rapid microwave- assisted extraction (MAE) procedure was developed and optimized for the isolation of borneol and bornyl acetate from Ferulago macrocarpa. Ferulago macrocarpa (Apiaceae) is an endemic plant in Iran. Experimental: Dried powdered aerial parts of plants were soaked in water and irradiated with microwave in a Clevenger extraction approach under optimum conditions (520 W and 0.5 h). Results & Discussion: Compared to traditional heating methods by Clevenger, the present method is more efficiency and proceeds in a short time. The isolation rates of borneol and bornyl acetate increased by about 2 and 1.3, respectively, as compared to the conventional thermal methods. Low cost, easy extraction, high purity of the extracted products and high efficiency are the advantages of this method. Furthermore, solvent free extraction of the essential oils is a good method for the development of green chemistry. Recommended applications/industries: The essential oil of this plant obtained by MAHD and HD methods, contained bornyl acetate as the most abundant component. The extracted essential oil by MAHD was qualitatively similar to that obtained by HD however; it was achieved in a much short time. Therefore pilot microwave reactor might be proven suitable for large scale extraction with potential industrial applications.

Keywords

Article Title [Persian]

جداسازی برنئول و برنیل استات از Ferulago macrocarpaبا استفاده از تابش ریزموج

Authors [Persian]

  • ژیلا اصغری
  • محمد صالحی
  • محسن مظاهری تهرانی
گروه شیمی، دانشگاه گلستان، گلستان، ایران
Abstract [Persian]

مقدمه و هدف: یک روش ساده و سریع برای استخراج بورنئول و بورنیل­استات از گیاهFerulago macrocarpa تحت تابش اشعه ریز­موج در شرایط بهینه می­باشد این گیاه بومی جنگلهای ایران است.
روش تحقیق: در این روش، اندام هوایی گیاه پس از خشک و پودر­کردن، در آب خیسانده شدند و سپس تحت تابش اشعه ریزموج در سیستم کلونجر در شرایط بهینه (520 وات و 5/0 ساعت) قرار گرفته شدند.
نتایج و بحث: نتایج این روش در مقایسه با روش حرارتی معمولی موثرتر بود و در مدت زمان کوتاهی عمل عصاره گیری انجام شد. سرعت استخراج دو ترکیب بورنیل و بورنیل­استات به ترتیب حدود 2 و 3/1 برابر بیشتر از روش معمولی بود. پایین بودن هزینه، بالا بودن بازده، کیفیت و خلوص بالای محصول، از مزایای این روش نسبت به روش حرارتی معمولی می­باشد. به علاوه به دلیل عدم استفاده از حلال­های آلی، در توسعه شیمی سبز و سازگار با محیط زیست حائز اهمیت می­باشد.





توصیه کاربردی/ صنعتی: میزان روغن این گیاه با استفاده از روش استخراج به کمک تابش اشعه ریز­موج و روش حرارتی معمولی غنی از بورنیل استات می­باشد. روغن استخراج شده با تابش ریز­موج از لحاظ کیفی مشابه با روش حرارتی معمولی بود که در زمان کوتاه تر انجام می­شود. بنابر­این استفاده از راکتور ریز موج می­تواند برای استخراج در مقیاس صنعتی مناسب باشد.

Keywords [Persian]

  • Ferulago macrocarpa برنئول برنیل استات

References

Buchbauer, G., Jäger, W., Jirovetz, L., Meyer, F. and Dietrich, H. 1992. Effects of valerian root oil, borneol, isoborneol, bornyl acetate and Isobornyl acetate on the motility of laboratory animals (mice) after inhalation. Pharmazie., 47: 620-622.

Chun-Jie W., Qin-wan H., Hong-yI Q., Ping G. and Shi-xiang, H. 2006. Promoting effect of borneol on the permeability of puerarin eye drops and timolol maleate eye drops through the cornea in vitro. Pharmazie., 61: 783-788.

Clark, A.M. 1996. Natural products as a resource for new drugs. Pharmaceutical Research., 13: 1133-1144.

Djouahri, A., Boudarene, L. and Meklati, E.Y., 2013. Effect of extraction method on chemical composition, antioxidant and anti-inflammatory activities of essential oil from the leaves of Algerian Tetraclinis articulata (Vahl) Masters. Industrial Crops and  Products., 44: 32-36.

Ganzler K., Bati J. and Valko K., 1990.Effective sample preparation method for extracting biologically active compounds from different matrices by a microwave technique. Journal of Chromatography A., 520: 257-262.

Hadjiakhoondi A., Aghe L.N. and Etemadi R.. 1992. Chemical and biological study of essential oil of Ferulago macrocarpa (Fenzi) Boiss, Effects of valerian root oil, borneol, isoborneol, bornyl acetate and isobornyl acetate on the motility of laboratory animals (mice) after inhalation, Publisher: Bait al-Hikmah., 620-622.

Heywood, V.H. 1985. Flowering Plants of the Word, Croom Helm. London 219-221.

Hao, J., Han, W., Huang, S., Xue, B. and Deng, X. 2002. Microwave- assisted extraction of artemisinin from Artemisia annua L. Separation and Purification Technology.,., 28: 191-196.

Kaufmann, B. and Christen, P. 2002. Recent extraction techniques for natural products: microwave-assisted extraction and pressurised solvent extraction.  Phytochemistry Analysis., 13: 105–113.

Lucchesi, M.E., Chemat, F. and Smadja, J. 2004. Solvent- free microwave extraction of essential oil from aromatic herbs: comparison with conventional hydro-distillation.  Journal of Chromatography A., 1043: 323- 327.

Lucchesi, M.E., Smadja, J., Bradshaw, S. Louw, W. and Chemat, F. 2007. Solvent free microwave extraction of Elletaria cardamomum L.: A multivariate study of a new technique for the extraction of essential oil. Journal of Food Engineering., 79: 1079-1086.

Meirong, L., Shaomei, Y., Yingqing, Z., Weiping, Q. and Meirong, F. 1993. The effect of borneol on evan's blue staining of rabbit and rat brain tissue. Institute of Clinical Pharmacology. 4.

Mohamed, A., Ferhat, A., Brahim, Y., Meklati, A., Jacqueline, Smadja B. and Farid Hemat, C. 2006. An improved microwave Clevenger apparatus for distillation of essential oils from orange peel. Journal of Chromatography A., 1112: 121–12.

Mozaffarian, V.1994.Plant Systematics, Vol. 2, Nashr Danesh Emrouz, Tehran 372.

Mozaffarian, V. 1996. A Dictionary of Iranian Plant Names, Farhang Moaser, Tehran 194-195.

Mozaffarian, V. 1983. The family of Umbelliferae in Iran, Key and distribution, Research Institute of Forests and Rangelands, Tehran, Iran, 35, 387.

Wang, Z.,  Ding, L.,  Li, T., Zhoua, X., Wanga, L. and Zhang, H. 2006 Improved solvent-free microwave extraction of essential oil from dried Cuminum cyminum L. and Zanthoxylum bungeanum Maxim. Journal of Chromatography A., 1102: 11–17.

Xiao-Jing, H., Qing-jie, L. and Yu-lan, L. 2006. Effects of Borneol injection on inflammation in focal cerebral ischemia reperfusion rats. West China Journal of Pharmaceutical Sciences, 21:523-526.

Xue, L., Chen, X.W., Fan, H.X., Fan, Sh., Liang, Y., Xia, J.X., Zhou, H. and Hu, Zh. 2006. Effect of borneol on levels of monoamine neurotransmitters in prefrontal cortex of rat after long-term continuous operations, Acta Academiae Medicinae Militaris Tertiae, 18.

 

Journal of Herbal Drugs (An International Journal on Medicinal Herbs)

Volume 4, Issue 2
Summer 2013
Pages 89-94

Files

Share

How to cite

Statistics