Effect of Ferulago angulata sub. carduchorum on total serum antioxidant activity and some of the humoral immune responses in broiler chicks

Document Type: Original article


1 Department of Animal Science, Khorasgan Branch, Islamic Azad University, Isfahan, Iran.

2 Department of Animal Science, Khorasgan Branch, Islamic Azad University, Isfahan, Iran;


Background & Aim: Ferulago angulata sub. carduchorum (locally name: Chavir or Chavil) is an important medicinal plant in Iran. The powder of aerial parts of this plant contains variety of components with different therapeutical effects such as antioxidant activities (AOA). This study was conducted to investigate the effect of dietary supplementation powder of aerial parts of this plant on total activities of serum antioxidant and some of the humoral immune responses in broiler chicks.
Experimental: Three hundred of 16 days-old commercial chicks (Ross 308) were randomly allocated to six treatments with five replicates. The dietary treatments consisted of the basal diet as control (A), 100 ppm (150 IU) of vitamin E (B), 70 g / 100 kg Flavofosfolipol (C), 0.3% (D), 0.6% (E), and 1% (F) of the powder of the aerial parts of this plant added to the basal diet. At 25 days, from each treatment of experiment, two chicks were randomly selected and sheep red blood cell (SRBC) was injected through their wings veins. At 30 days, blood samples (ten sample of per treatment) for the test of antibody measurement produced against SRBC, the influenza virus and Newcastle were prepared. Moreover, with the blood extension and numbering of Hetrophil and Lymphocyte calculated and hetrophil to lymphocyte ratio were estimated. At 42 days, blood samples (ten samples per treatment) were taken for measuring total activities of serum antioxidant.
Results & Discussion: Results indicated that supplementing of 0.6% of F. angulata significantly increased total activities of serum antioxidant compared to control birds (p ≤ 0.05). There is no significant difference among the treatments of experiment in the number of Hetrophil, Lymphocyte their ratio and the antibody against the virus of Newcastle and SRBC. However, this antibody against the influenza virus at the treatment of 3 g/kg F. angulata in comparison to control treatment was more.
Recommended applications/industries: Use of F. angulata in broiler diet could be increase total serum antioxidant activity and improve humoral immunity.


Article Title [Persian]

اثر گیاه چویل بر فعالیت آنتی اکسیدانی سرم و برخی واکنش های ایمنی در جوجه های گوشتی

Authors [Persian]

  • رضا گواهی 1
  • غلامرضا قلمکاری 1
  • مجید طغیانی 1
  • شهین اقبال سعید 1
  • محمد محمدرضایی 1
  • مهدی شهریاری 1
  • علیرضا دهقانی ابری 2
1 دانشگاه آزاد اسلامی واحد خوراسگان، اصفهان، ایران.
2 دانشگاه آزاد اسلامی واحد خوراسگان، اصفهان، ایران.
Abd El – Motaal, A.M., Ahmed, A.M.H., Bahakaim, A.S.A, Fathi, M.M., 2008. Productive performance and immune competence of commercial laying hens given diets supplemented with Eucalyptus. International Journal of Poultry Science., 7 (5): 445-449.

Al-Ankari, A.S., Zaki, M.M., Sultan, S.I., 2004. Use of habek mint (Mentha longifolia) in broiler chicken diets. International Journal Poultry Science., 3(10): 629-634.

Botsoglou, N.A., Florou-Paneri, P., Christaki, E., Fletouris, D.J., Spais, A.B., 2002. Effect of dietary oregano essential oil on performance of chickens and on iron-induced lipid oxidation of breast, thigh and abdominal fat tissues. British Poultry Science., 43: 223-230.

Cosention, S., Tuberoso, S.I., Pisano, B., 1999. In vitro antimicrobial activity and chemical composition of Sardinian Thymus essential oils. Letters in  Applied Microbiology, 29: 130-135.

Davison, T.F., Rowell, L.G., Rea J., 1983. Effects of dietary corticosterone on peripheral blood lymphocyte and granulocytes populations in immature domestic fowl. Research in Veterinary Science., 34: 236-239.

Farag, R.S., Badei, A.Z.M.A., Hewedi, F.M., El- Baroty, G.S.A., 1989. Antioxidant activity of some spice essential oils on linoleic acid oxidation in aqueous media. Journal American Oil Chemical Society., 66: 792-799.

Ghalamkari, G., Landy, N., Toghiani, M., Modaresi, M., Ghalamkari, Z., 2011. Efficiency of Echinacea purpurea on total antioxidant activity in serum of broiler chicks. The 9th International Conference on Food Engineering and Biotechnology, Bangkok, Thailand. 

Ghasempour, H.R., Shirinpour, E., Heidari, H., 2007. The constituents of essential oils of Ferulago angulata (Schlecht) Boiss at two different habitats, Nevakoh and Shahoo, Zagross Mountain, Western Iran. Iranian Journal of Science and Technology., Transaction A, 31(A3).

Gross, W.B., Siegel, P.B., 1983. Evaluation of the heterophil / lymphocyte ratio as a measure of stress in chickens. Avian Disease., 27: 972-979.

Hosseni Mansob, N., Nezhady, M.M., 2011. The effect of using thyme, garlic and nettle on performance, carcass quality and blood parameters. Annale of Biological Research., 4: 315-320.

Huda-Faujan, N., Noriham, A., Norrakiah, A.S., Babji, A.S., 2009. Antioxidant activity of plants methanolic extracts containing phenolic compounds. African Journal of Biotechnology., 8: 484-489.

Isabel, B., Santos, Y., 2009. Effects of dietary organic acid and essential oils on growth performance and carcass characteristics of broiler chickens. Journal Apply Poultry Research., 18: 472-476.

Kabir, S.M.L., Rahman, M.M., Rahman, M.B., Ahmad, S.U., 2004. The dynamics of probiotics on growth performance and immune response in broilers. International Journal Poultry Science., 3: 361-364.

Khanavi, M., Hajimahmoodi, M., Cheraghi-Niroomand, M., Kargar, Z., Ajani, Y., Hadjiakhoondi, A., Oveisi, M.R., 2009. Comparison of the antioxidant activity and total phenolic contents in some Stachys species. African Journal of Biotechnology., 8: 1143-1147.

Lis–Balchin, M., Deans, G., Stanley., Eaglesham, E., 1998. Relationship between bioactivity and chemical composition of commercial essential oils. Journal Flavour and Fragrance., 13: 98-104.

Lopez-Bote, C.J., Gray, J.I., Gomma, E.A., Flegal, C.J., 2010. Effect of dietary administration of oil extracts from rosemary and sage on lipid oxidation in broiler meat. British Poultry Science., 39: 235-240.

Mathivanan, R., Kalaiarasi, K., 2007. Panchagavya and Andrographis panculata as alternatives to antibiotic growth promoters on haematological, serum biochemical parameters and immune status of broilers. Journal Poultry Science., 44: 2. 198-204.

Maxwell, M.H., 1993. Avian blood leukocyte responses to stress. World’s Poultry Science Journal., 49: 34-43.

Ramarao, S.V., Reddy, M.R., Raju, M.V.L.N., Panda, A.K. 2004. Growth, nutrient utilization and immune competence in broiler chicken fed probiotic, gut acidifier and antibacterial compounds. Indian Journal Poultry Science., 39: 125-130.

Rath, N.C., Huff, W.E., Huff, G.R., 2006. Effects of humic acid on broiler chickens. Poultry Science., 85: 410-414.

Roofchaee, A., Irani, M., Ebrahimzadeh, M.A., Akbari, M.R., 2011. Effect of dietary oregano (Origanum vulgare L.) essential oil on growth performance, cecal microflora and serum antioxidant activity of broiler chickens. African Journal of Biotechnology., 10: 6177-6183.

Ross Company., 2007. Ross 308 broiler Performance Objectives, Alabama, USA: Aviagen, page, 24.

SAS Institute., 2008. SAS User's guide version 9.02 review edition, Cary NC: SAS Institute Inc, 176

Taran, M., Ghasempour, H.R., Shirinpour, E., 2010. Antimicrobial activity of essential oils of Ferulago angulata subsp. Carduchorum. Jundishapur Journal of Microbiology., 3(1): 10-14.

Thrall, M.A., 2004. Veterinary Hemotalogy and Clinical Chemistry. Lippincott Williams & Wilkins, pp.10.

Vossen, E., Ntawubizi, M., Ntawubizi, K., Raes, K., Smet, G., Uyghebaert, S., Arnouts, S., De Smet., 2011. Effect of dietary antioxidant supplementation on the oxidative status of plasma in broilers. Journal of Animal Physiology and Animal Nutrition., 95: 198–205.

Yesilbag, D., Eren, M., Agel, H., Kovanlikaya, A., Kovanlikaya, F., 2011. Effects of dietary rosemary, rosemary volatile oil and vitamin E on broiler performance, meat quality and serum sod activity. British Poultry Science., 52: 472-482.