Effect of garlic (Allium sativum L.) extract on some heamatological parameters and immune response of rainbow trout fingerlings

Document Type : Original article


1 Department of Fisheries, Science and Research Branch, Islamic Azad University, Khouzestan, Iran

2 Department of Fisheries, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran


Background & Aim: Continuous use of antibiotics leads to drug resistance and thereby to a reduced efficacy of the drugs. Antibiotics accumulate in the environment and fish pose a potential risk to consumers and to the environment.Increased public awareness of the negative effects caused by overexposure to synthetic chemicals has led to the search for ‘‘green drugs’’, such as organic and synthetic chemical-free food products. The main goal this work was effect of Allium sativum on some parameters and immune response of rainbow trout fingerlings. Experimental: A 500 fish with weight 10.47±0.29 g were purchased from a fish farm of Yasoj, Southern Iran. In this study, 15 fiberglass tanks (300 L) were used. Experimental treatments were different levels of  A. satinum extract included 0.05, 0.1, 0.15, and 0.2 g/kg  that were added to the diet. Control group was no add supplement to the diet. In final, biochemicals and hematologals were analyzed.  Results & Discussion: Results indicated that 0.05 g/kg garlic extract no had on some paramteres, including RBC, hematocrite percent, hemoglobin and white blood cell, while other experimental groups  had significant effects on the paramters serum. The most of albomin and globulin were obtined from 0.05 g/kg garlic extract trteatment. The highest of lysozyme activity was observed in 0.1 g/kg garlic extract and the lowest  lysozyme activity was obtined from control group. Recommended applications/industries: The obtained results indicated that A. sativum extract had immunostimulatory effect on these immunological variables of fingelings of rainbow trout and the best levels of extract effect 0.15 g/kg is recommended.


Article Title [فارسی]

تأثیر عصاره گیاه سیر (Allium sativum L.) بر برخی شاخص های خونی و ایمنی ماهی قزل آلای انگشت قد

Authors [فارسی]

  • سید پرویز دادوران 1
  • منصوره قائنی 2
  • ابوالفضل عسکری ساری 2
1 گروه شیلات، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، خوزستان، ایران
2 گروه شیلات، واحد اهواز، دانشگاه آزاد اسلامی، اهواز، ایران
Abstract [فارسی]

مقدمه و هدف: استفاده بی رویه و مداوم از آنتی بیوتیک­ها منجر به مقاومت دارویی و بنابراین کاهش اثرات آن­ها می شود. تجمع آنتی بیوتیک ها در محیط آب و بدن ماهی، برای مصرف کننده و محیط زیست خطراتی را به همراه خواهد داشت. افزایش آگاهی عمومی از تأثیرات منفی مواد شیمیایی مصنوعی سبب شد تا تحقیقات به سمت استفاده از گیاهان دارویی مانند مواد طبیعی و بدون مواد شیمیایی سوق پیدا کند. هدف این مطالعه تأثیر گیاه دارویی سیر بر روی برخی پارامترهای ایمنی ماهیان انگشت قد قزل آلا می باشد. روش تحقیق: به منظور بررسی تأثیر عصاره گیاه دارویی سیر (Allium sativum) بر روی شاخص های خونی و ایمنی غیر اختصاصی ماهیان قزل آلای انگشت قد، تعداد 500 عدد ماهی با وزن 29/0±47/10 گرم از مزارع پرورشی ماهی قزل آلا در یاسوج خریداری گردید. مخازن مورد استفاده در آزمایش، شامل 15 عدد تانک فایبرگلاس 300 لیتری دایره ای شکل بود. به منظور بررسی اثر درصدهای مختلف عصاره سیر در جیره، 5 تیمار مختلف از عصاره سیر، با سطوح (05/0، 1/0، 15/0 و 2/0 گرم بر کیلوگرم) به جیره اضافه شد و تیمار شاهد فاقد هرگونه مکمل افزودنی بود. آزمایش در سه تکرار به ازای هر تیمار انجام شد. در پایان فاکتورهای بیوشیمیایی و هماتولوژیک مورد بررسی قرار گرفتند. نتایج و بحث: نتایج آزمایش نشان داد که میزان RBC، درصد هماتوکریت، غلظت هموگلوبین و گلبول های سفید خون در گروه 1 اختلاف معنی داری با گروه کنترل نداشت (05/0p ≥ ). از طرفی اگرچه بین گروه 2، 3 و 4 اختلاف معنی داری وجود نداشت (05/0p ≥ )، اما در این گروه ها با گروه کنترل تفاوت معنی دار مشاهده گردید (05/0p ≤). بیشترین میزان آلبومین و گلوبولین نیز در تیمار سوم مشاهده گردید. بیشترین میزان فعالیت لیزوزیم در گروه 2 و کمترین متعلق به گروه کنترل بود. توصیه کاربردی/ صنعتی: با توجه به نتایج حاصل از این آزمایش می توان اظهار داشت که عصاره سیر توانسته بر برخی از شاخص های ایمنی در ماهی قزل آلای رنگین کمان انگشت قد تأثیر مثبتی داشته باشد و بهترین سطح این عصاره جهت بهبود شاخص های ایمنی g/kg 15/0 پیشنهاد می شود.

Keywords [فارسی]

  •  گیاه سیر  سیستم ایمنی  قزل آلای رنگین کمان
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Volume 4, Issue 4 - Serial Number 4
Special issue: Full text in Farsi and Abstract in English
Winter 2014
Pages 162-169
  • Receive Date: 20 December 2013
  • Revise Date: 08 January 2014
  • Accept Date: 09 February 2014