Antibacterial activity of different extracts from the aerial parts and roots of Stachys lavandulifolia Vahl harvested at two stages of growth

Document Type : Original article

Authors

1 Department of Botany, Marand Branch, Islamic Azad University, Marand, Iran

2 Department of Microbiology, Maragheh Branch, Islamic Azad University, Maragheh, Iran

3 Department of Animal Science, Maragheh Branch, Islamic Azad University, Maragheh, Iran

Abstract

Background & Aim: Nowadays, the application of traditional medicine and herbal medicinal plants instead of chemical and synthetic drugs has captured the attention of the researchers. The purpose of this study was to investigate the antimicrobial effects of the aquatic, ethanolic and methanolic (80%) extracts of the aerial and root parts of Stachys lavandulifolia Vahl which were taken from the countryside of Marand (Eastern Azerbaijan, Iran) in two generative and vegetative phases against standard strains of microorganisms. Experimental: Antimicrobial activity of the alcoholic and aquatic extracts from different parts of S. lavandulifolia was investigated against standard strains of Staphylococcus aureus (ATCC 6538), Escherichia coli (ATCC 8739), Pseudomonas aeruginosa (ATCC 9027) through well-plate and micro-dilution methods. Ampicillin as antibacterial agent was used as positive control. Results & Discussion: Results of antimicrobial properties of S. lavandulifolia extracts revealed that the aquatic extract of the plant had no antibacterial effects. While the ethanolic and methanolic (80%) extracts showed the growth inhibition effect against standard strains of examined microorganisms. Their effect against Gram-positive bacteria was higher than Gram-negative bacteria. The antimicrobial activity of the ethanolic extract from the inflorescence of S. lavandulifolia was higher than the alcoholic extract of the roots. Recommended applications/industries: The overall results indicated the ethanolic extract of the inflorescence of Stachys lavandulifolia has the best antimicrobial effects and it can be employed in manufacturing desirable bactericidal agents.

Keywords

Article Title [فارسی]

اثر ضدباکتریایی عصاره های اندام هوایی و ریشه چای کوهی (Stachys lavandulifolia Vahl) در دو مرحله رشد

Authors [فارسی]

  • مسعود طاهری 1
  • سامان مهدوی 2
  • یوسف مهمان نواز 3
1 گروه گیاهشناسی، دانشگاه آزاد اسلامی واحد مرند، ایران
2 گروه میکروبیولوژی، دانشگاه آزاد اسلامی مراغه، ایران
3 گروه علوم دامی؛ دانشگاه آزاد اسلامی مراغه، ایران
Abstract [فارسی]

مقدمه و هدف: امروزه جایگزینی داروهای طبیعی و مورد استفاده در طب سنتی به جای داروهای شیمیایی و سنتزی مورد توجه اغلب پژوهشگران قرار گرفته است. هدف از تحقیق حاضر مطالعه اثرات ضدمیکروبی عصاره های آبی، الکلی و متانولی اندام هوایی و ریشه گیاه گل سنبله ای با نام علمی Stachys lavandulifolia Vahl در دو مرحله رویشی جمع آوری شده از مرند استان آذربایجان شرقی بود.
مواد و روش: فعالیت ضدمیکروبی عصاره های مختلف اندام هوایی و ریشه گیاه گل سنبله ای با نام علمی Stachys lavandulifolia Vahl جمع آوری شده در دو مرحله رویشی علیه Staphylococcus aureus (ATCC 6538), Escherichia coli (ATCC 8739), Pseudomonas aeruginosa (ATCC 9027) به روش رقت سازی مورد مطالعه قرار گرفت:
نتایج و بحث: نتایج خاصیت ضد میکروبی عصاره گل سنبله ای نشان داد که عصاره آبی گیاه مذکور فاقد اثر ضدمیکروبی در حالی که عصاره های الکلی 80 درصد متانول و اتانول دارای خاصیت ضدمیکروبی موثری بودند. خصوص این اثر ضدباکتریایی بر باکتری های گرم مثبت بیشتر بود. همچنین خاصیت ضدباکتریایی گل آذین گیاه گل سنبله ای بیشتر از اندام ریشه بود.
توصیه صنعتی /کابربردی: باتوجه به نتایج حاصل، عصاره الکلی سرشاخه های گل سنبله ای دارای خاصیت ضدبکتریایی قابل ملاحظه ای بود که می توان از آن به عنوان یک ضدمیکروب طبیعی استفاده نمود.

Keywords [فارسی]

  •  Stachys lavandulifolia Vahl خاصیت ضدمیکروبی عصاره الکلی

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History

  • Receive Date: 21 June 2014
  • Revise Date: 30 June 2014
  • Accept Date: 01 July 2014

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