In vitro anti-fungal activity of watercress (Nasturtium officinale) extract against Fusarium solani, the causal agent of potato dry rot

Document Type: Original article

Authors

1 Plant Protection Research Department, Agricultural and Natural Resources Research Centre of Hamadan, Hamadan, Iran

2 University of Applied Science and Technology of Hamadan, Hamadan, Iran

Abstract

Background & Aim: The aim of this study was to assess the antifungal effect of the watercress extract on the growth of Fusarium solani, the causal agent of potato dry rot.
Experimental:  This research was done in a completely randomized design with three concentrations and four replicates on potato dextrose agar (PDA) culture medium.
Results: Results of this study indicated that all concentrations of alcoholic extract of watercress can significantly inhibit mycelia growth of Fusarium solani on PDA medium. Among different concentrations of watercress extract, the highest inhibition zone of the fungus (26.1 mm in diameter) was obtained at dose of 600 mg/ml. The rate of growth inhibition was concentration dependent. However, no significant difference in the rate of growth inhibition of the fungus by watercress extract was seen between the 500 mg/ml and 400 mg/ml or between 400 mg/ml and 300 mg/ml of the plant extract.
Recommended applications/industries: The overall results of this experiment indicated that the alcoholic extract of watercress can inhibit mycelia growth of Fusarium solani in a dose dependent manner. It seems therefore, that the watercress extract has the potential to be employed for manufacturing natural fungicidal compounds. It is recommended that the experiment to be tested on potato tubers (in vivo conditions).

Keywords


Article Title [Persian]

فعالیت ضد قارچی عصاره گیاه دارویی علف چشمه (Nasturtium officinale) علیه قارچ Fusarium solani عامل پوسیدگی خشک سیب زمینی

Authors [Persian]

  • جعفر نیکان 1
  • هادی خاوری 2
1 گروه حفظ نباتات، مرکز تحقیقات کشاورزی و منابع طبیعی استان همدان
2 دانشگاه کاربردی و صنعتی، همدان، ایران
Abstract [Persian]

مقدمه و هدف: پوسیدگی ناشی از گونه های مختلف فوزاریوم (Fusarium spp.) ، یکی از بیماری های قارچی مهم پس از برداشت سیب زمینی است. مهمترین روش های مبارزه با این بیماری شامل حمل و نقل و انبارداری مناسب غده های سیب زمینی به منظور به حداقل رساندن و ترمیم زخم های روی غده ها و نیز سم­پاشی غده ها با ترکیبات قارچ کش است. اگر چه مدیریت موثر آفات با کاربرد آفت کش های مصنوعی به دست می آید ولی به دلیل افزایش آگاهی از خطرات مرتبط با استفاده از این مواد شیمیائی، توجه زیادی بر روی روش های جایگزین مانند کاربرد ترکیبات طبیعی گیاهان معطوف گشته است.
روش تحقیق: در این مطالعه چهار غلظت مختلف از عصاره الکلی علف چشمه  (Nasturtium officinale R.Br.) برای فعالیت ضد قارچی علیه Fusarium solani  عامل اصلی پوسیدگی خشک سیب زمینی در استان همدان در شرایط درون شیشه (روی محیط کشت) مورد ارزیابی قرار گرفت. ملاک ارزیابی درصد ممانعت از رشد قارچ بود.
نتایج و بحث:نتایج نشان داد که همه غلظت های مورد استفاده از عصاره الکلی علف چشمه در کنترل قارچ بر روی محیط کشت موثرند. بالاترین میزان ممانعت از رشد قارچ به ترتیب با 7/ 32% و 1/ 26%  با استفاده از تیمار قارچ کش و غلظت mg/m l600  عصاره علف چشمه به دست آمد.  به علاوه میزان ممانعت از رشد قارچ با عصاره وابسته به غلظت بود. به هر حال از نظر درصد ممانعت از رشد قارچ عامل بیماری تفاوت معنی داری بین غلظت های  mg/ml500 و mg/ml400  یا بین غلظت­های mg/ml400 و mg/ml300 عصاره علف چشمه مشاهده نشد.





توصیه کاربردی/ صنعتی: با توجه به نتایج این آزمایش به نظر می رسد که عصاره علف چشمه توانائی کاربرد به عنوان قارچ کش طبیعی علیه قارچF.solaniرا دارد. پیشنهاد می شودکه این عصاره در شرایط طبیعی (روی غده های سیب زمینی ) نیز اجرا گردد.

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