Effects of different medium on morphophysiological traits of Lippia citriodora H.B.K. during in vitro culture condition

Document Type: Original article


1 Department of Horticulture, College of Agriculture, Tehran Science and Research branch, Islamic Azad University, Tehran, Iran;

2 Research Institute of Forests and Rangelands, Tehran, Iran;

3 National Institute of Genetic Engineering and Biotechnology, Tehran, Iran;

4 Department of Plant Sciences, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran;


Background & Aim: Lemon verbena (Lippia citriodora H.B.K.) is a richly scented herb and has a long history as a sacred and medicinal plant. Select an appropriate medium in tissue culture is a necessity for success Experimental: To evaluate the effect of 10 medium of previous researches for Lippia micropropagation (1- MS medium supplemented with BAP (3 mgL-1) + IBA (0.1 mgL-1) + activated charcoal (1 gL-1) 2- MS medium supplemented with BAP (1 mgL-1) + IBA (0.5 mgL-1) + activated charcoal (1 gL-1) 3- 1/2MS 4- MS medium supplemented with BAP (5 mgL-1) 5- MS medium supplemented with BAP (1 mgL-1) 6- MS medium supplemented with activated charcoal (1 gL-1) 7- MS medium supplemented with BAP (1 mgL-1) + activated charcoal (1 gL-1) 8- MS medium supplemented with BAP (1 mgL-1) + AgNO3 (5 mgL-1) 9- MS medium supplemented with AgNO3 (5 mgL-1) 10- MS (control)), an experiment was conducted as a Completely Randomized Design (CRD) with three replicates and three explants per replication.  Results & Discussion: The results showed positive effects for BAP (5 mgL-1) and somewhat active charcoal, but contrary to predictions, AgNO3 had no effect on reducing senescence and leaf necrosis. Recommended applications/industries: Therefore, Based on the finding, the four medium (MS medium supplemented with BAP (5 mgL-1)) with the highest rates of shoot fresh weight, leaf number, internodes number, shoot number, root fresh weight, callus diameter and root dry weight can be introduced as a suitable medium of tissue culture in this experimental conditions.


Article Title [Persian]

اثر محیط های مختلف کشت بر برخی صفات مورفوفیزیولوژیک به لیمو در شرایط کشت درون شیشه ای

Authors [Persian]

  • حسن نورافکن 1
  • فاطمه سفیدکن 2
  • سیدامیر موسوی 3
  • مظفر شریفی 4
  • احمد خلیقی 1
1 گروه علوم باغبانی، دانشکده کشاورزی، واحد علوم و تحقیقات، تهران، ایران؛
2 موسسه تحقیقات جنگل ها و مراتع کشور، تهران، ایران؛
3 پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری، تهران، ایران
4 گروه علوم گیاهی، دانشکده علوم زیستی، دانشگاه تربیت مدرس، تهران، ایران؛
Abstract [Persian]

مقدمه و هدف: به­لیمو با نام علمی Lippia citriodora H.B.K سال­هاست به عنوان یک گیاه دارویی و معطر کشت شده و مورد مصرف قرار می‌گیرد. تکثیر جنسی به­لیمو به علت عدم جوانه­زنی بذر معمول نیست و تکثیر آن غیرجنسی انجام می­شود. انتخاب محیط کشت مناسب یکی از ضرورت­های موفقیت در کشت بافت است. روش تحقیق: به منظور ارزیابی اثرات 10 محیط کشت مورد استفاده در تحقیقات کشت بافت (1- MS حاوی BAP (3 mgL-1) + IBA (0.1 mgL-1) + زغال فعال (1 gL-1)، 2- MS حاوی BAP (1 mgL-1) +IBA (0.5 mgL-1) + زغال فعال (1 gL-1)، 3- 1/2MS، 4- MS حاوی BAP (5 mgL-1)، 5- MS حاوی BAP (1 mgL-1)، 6- MS حاوی زغال فعال (1 gL-1)، 7- MS حاوی BAP (1 mgL-1) + زغال فعال (1 gL-1)، 8- MS حاوی BAP (1 mgL-1) + نیترات نقره (5 mgL-1)، 9- MS حاوی نیترات نقره (5 mgL-1) و 10- شاهد (MS)) بر صفات مورفوفیزیولوژیک به‌لیمو، آزمایشی در قالب طرح کاملاً تصادفی با سه تکرار و سه ریزنمونه در هر تکرار انجام شد. نتایج و بحث: نتایج اثرات مثبت BAP (5 mgL-1) و تا حدودی زغال­فعال را بر بسیاری از شاخص­های رشد نشان داد ولی برخلاف انتظار، نیترات­نقره تاثیری روی کاهش پیری و نکروز برگ نشان نداد. توصیه کاربردی/ صنعتی: با توجه به یافته­ها می­توان محیط کشت چهارم (MS حاوی BAP (5 mgL-1)) را با داشتن بالاترین میزان از شاخص­های وزن تر شاخساره، تعداد برگ، تعداد میانگره، تعداد شاخه، وزن تر ریشه، قطر کالوس و وزن خشک ریشه به عنوان محیط مناسب کشت بافت در شرایط آزمایشی اشاره شده انتخاب و معرفی نمود.

Keywords [Persian]

  • به لیمو بنزیل آدنین کشت بافت گیاهان دارویی BAP

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