Comparative polyphenol contents, free radical scavenging properties and effects on adipogenesis of Chorisia Chodatii and Chorisia Speciosa

Document Type: Original article

Authors

1 Pharmacognosy Department, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt

2 Pharmacognosy Department, Faculty of Pharmacy, Assiut University, 71515 Assiut, Egypt

3 Alliance for Research on North Africa (ARENA), Tsukuba University, 305-8572 Tsukuba, Japan; Graduate School of Life and Environmental Sciences, Tsukuba University, 305-8572 Tsukuba, Japan

Abstract

Background & Aim: Chorisia is an important Bombacaceous plant that is traditionally used for a variety of ailments. Due to its richness in several bioactive phytocompounds, some Chorisia species showed a wide range of important biological effects. Accordingly, the present work was undertaken to comparatively evaluate the antioxidant potential and effects on adipogenesis of Chorisia chodatii Hassl. and Chorisia speciosa A. St.-Hil. in relation to their phenolic contents.  Experimental: Total polyphenol contents and free radical scavenging potentials of the total ethanol extracts of leaves, flowers, fruits and seeds of both species, in addition to four main fractions of their leaf and flower extracts, were evaluated using the Folin-Ciocalteu's method and the DPPH assay, respectively. Besides, their effects on adipogenesis were studied using the 3T3-L1 preadipocytes model. A phytochemical screening of their different phytoconstituents was also considered.  Results & Discussion: Total ethanol extracts along with their successive fractions of various parts of both species caused a concentration-dependent induction of 3T3-L1 preadipocytes differentiation, but with a noticeable reduction of the size of the lipid droplets at the lower concentrations 5 and 10 µg/ml. In addition, these extracts showed a strong evidence of their richness in free radical scavengers. The ethyl acetate, aqueous and chloroform fractions of different plant parts exhibited the greatest effects on adipogenesis, substantial free radical scavenging properties and the highest polyphenol contents, respectively. Industrial and practical recommendations: Results collectively revealed that the observed effects of both Chorisia species on adipogenesis as well as their anti-radical properties are positively related to their pool of flavonoids and other phenolics. They also suggest their potential value in obesity-related disorders and for prevention of free radical mediated diseases. Further studies for investigating the molecular basis of their effects on adipogenesis accompanied by detailed phytochemical analysis, especially of their polar and flavonoids-rich extracts, will also be strongly recommended.

Keywords


Article Title [Persian]

مقایسه ترکیبات پلی فنلی، خاصیت مهارکنندگی رادیکالهای آزاد و تاثیر بر آدیپوژنز در Chorisia Chodatii و Chorisia Speciosa

Authors [Persian]

  • جون رفعت 1
  • ثمر یهیا دسوکی 1
  • محمد احمد رامادان 2
  • محمد صالح کامل 1
  • جونکیو هان 3
  • هیرکو ایسودا 3
1 گروه داروسازی، دانشکده داروسازی، دانشگاه Minia، مصر
2 گروه داروسازی، دانشکده داروسازی، دانشگاه Assiut، مصر
3 اتحادیه تحقیقات شمال آفریقا (ARENA)، دانشگاه تسوکوبا، تسوکوبا، ژاپن. دانشکده تحصیلات تکمیلی زندگی و علوم محیط زیست، دانشگاه تسوکوبا، تسوکوبا، ژاپن
Abstract [Persian]

مقدمه و هدف: Chorisia گیاهی مهم از خانواده Bombacaceae است که به طور سنتی برای انواع بیماریها استفاده می شود. با توجه به غنی بودن آن از نظر برخی از ترکیبات فیتوشیمیایی زیست فعال، برخی از گونه های Chorisia طیف گسترده ای از اثرات مهم بیولوژیکی را نشان داده اند. بر این اساس، در مطالعه حاضر، به مقایسه پتانسیل آنتی اکسیدانی و اثرات آن بر آدیپوژنز در دو گونهChorisia chodatii و Chorisia speciosa بر اساس مقدار ترکیبات فنولی موجود در آنها پرداخته شده است. روش تحقیق: کل ترکیبات فنلی و پتانسیل مهار رادیکالهای آزاد در عصاره الکلی برگ، گل، میوه و دانه در دو گونه ذکر شده، و فراکشنهای اصلی در عصاره برگ و گل، به ترتیب با استفاده از روش فولین سیوکالتیو و DPPH مورد بررسی قرار گرفتند. به علاوه تاثیر آنها بر آدیپوژنز در مدل سیستمهای آدیپوسیتهای 3T3-L1 مورد ارزیابی قرار گرفت و بررسی ترکیبات مختلف فیتوشیمیایی آن نیز در نظر گرفته شد. نتایج و بحث: کل عصاره اتانولی همراه با فراکشن های به دست آمده از قسمتهای مختلف در هر دو گونه، باعث القای تمایز وابسته به غلظت در آدیپوسیتهای 3T3-L1 شد ولی در کمترین غلظت (µg/ml 10-5) باعث کاهش قابل توجه در اندازه ذرات چربی شد. به علاوه این عصاره ها تاثیرات قوی بر مهار رادیکالهای آزاد از خود نشان دادند. فراکشن های به دست آمده از بخشهای مختلف گیاه با استفاده از اتیل استات، آب و کلروفرم به ترتیب بیشترین تاثیر بر آدیپوژنز، مهار رادیکالهای آزاد و بیشترین مقدار ترکیبات فنلی را نشان دادند. توصیه های کاربردی/ صنعتی: نتایج به دست آمده در مجموع نشان داد که تاثیر هر دو گونه Chorisia بر آدیپوژنز و رادیکالهای آزاد ناشی از میزان قابل توجه فلاونوئیدها و ترکیبات فنلی در آنها است. به علاوه نتایج حاکی از ارزش بالقوه این دو گونه در پیشگیری از اختلالات مرتبط با چاقی و بیماریهای مرتبط با رادیکال های آزاد است. مطالعات بیشتر برای بررسی اساس مولکولی تاثیر آنها بر آدیپوژنز همراه با آنالیزهای فیتوشیمیایی دقیق به خصوص در عصاره های غنی از فلاونوئیدها و ترکیبات قطبی پیشنهاد می شود.

Keywords [Persian]

  • آدیپوژنز آنتی اکسیدان Bombacaceae Chorisia پلی فنل ها سلولهای T3-L1
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