Mardani-Nejad, S., Khavari-Nejad, R., Saadatmand, S., Najafi, F., Aberoomand Azar, P. (2015). Mechanisms of non-enzymatic antioxidant defense system of different organs of Catharanthus roseus for protective of cell membrane. Journal of Herbal Drugs
(An International Journal on Medicinal Herbs), 5(4), 219-224.
Shahin Mardani-Nejad; Ramazan Ali Khavari-Nejad; Sara Saadatmand; Farzaneh Najafi; Parviz Aberoomand Azar. "Mechanisms of non-enzymatic antioxidant defense system of different organs of Catharanthus roseus for protective of cell membrane". Journal of Herbal Drugs
(An International Journal on Medicinal Herbs), 5, 4, 2015, 219-224.
Mardani-Nejad, S., Khavari-Nejad, R., Saadatmand, S., Najafi, F., Aberoomand Azar, P. (2015). 'Mechanisms of non-enzymatic antioxidant defense system of different organs of Catharanthus roseus for protective of cell membrane', Journal of Herbal Drugs
(An International Journal on Medicinal Herbs), 5(4), pp. 219-224.
Mardani-Nejad, S., Khavari-Nejad, R., Saadatmand, S., Najafi, F., Aberoomand Azar, P. Mechanisms of non-enzymatic antioxidant defense system of different organs of Catharanthus roseus for protective of cell membrane. Journal of Herbal Drugs
(An International Journal on Medicinal Herbs), 2015; 5(4): 219-224.
Mechanisms of non-enzymatic antioxidant defense system of different organs of Catharanthus roseus for protective of cell membrane
1Department of Biology, Science and Research Branch, Islamic Azad University,Tehran,Iran
2Faculty of Biology Sciences, Kharazmi University,Tehran, Iran
3Department of Chemistry, Science and Research Branch, Islamic Azad University,Tehran,Iran
Abstract
Background & Aim: When the level of free radicals is increased and also when both the enzymatic systems and low molecular antioxidants are not sufficient to protect the organism, it seems necessary to get antioxidants from external sources. This study aimed to evaluate the antioxidant potential of different parts of Catharantus roseus Experimental: The antioxidant potential of ethanol extracts of roots, stems, leaves, flowers, seed pods and seeds of C. roseus plant were measured based on 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, inhibition of linoleic acid peroxidation by ferric thiocyanate (FTC), and malondialdehyde inhibition by thiobarbituric acid method (TBA) against the standards: Butylated hydroxytoluene (BHT), Butylated hydroxyanisole (BHA) and ascorbic acid. Results & Discussion: The phenolic content in the root (61.61 ± 2.58 µg of gallic acid per mg of dried weight extract) according to Folin–Ciocalteu method were more than any other organs. In scavenging a half of DPPH• free radicals (IC50), the extract of root, seed, and leaf (238.9 ± 2.02, 253.78 ± 1.42, 277.95 ± 2.56 µg mL-1),respectively, had the best performance. In the inhibition of linoleic acid peroxidation, the root extract had the best inhibitory power after ascorbic acid and BHT, and the leaf and seed extract had the performance similar to BHA. The root (78.68 ± 0.54%), and seed (77.44 ± 0.66%) had the best performance in the inhibition of MDA compared with other extracts. Pearson correlation coefficients between the phenolic content and antioxidant capacity of extracts were high and equal to -0.838, 0.895, and 0.740, respectively, according to DPPH, FTC and TBA methods. Industrial and practical recommendations: The results of this study can be promising in the potential of other applications of the plant organ especially in the inhibition of free radicals and lipids' peroxidation.
1گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
2دانشکده علوم زیست شناسی، دانشگاه خوارزمی، تهران، ایران
3گروه شیمی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
Abstract [Persian]
مقدمه و هدف: وقتی سطح رادیکال های آزاد افزایش می یابد و سیستم های آنزیمی و ملکول های کوچک آنتی اکسیدانی قادر به محافظت از موجود زنده نباشد، دریافت آنتی اکسیدان از منابع خارجی لازم به نظر می رسد. این مطالعه با هدف بررسی توان آنتی اکسیدانی قسمت های مختلف کاتارانتوس روزئوس انجام شد. مواد و روش ها: پتانسیل آنتی اکسیدانی عصاره های اتانولی ریشه ها، ساقه ها، برگ ها، گل ها، غلاف دانه ها و دانه های گیاه کاتارانتوس روزئوس بر اساس مهار رادیکال آزاد 1، 1-دی فنیل-2-پیکریل هیدرازیل (DPPH)، مهار پراکسیداسیون لیپیدی بوسیله روش فریک تیوسیانات (FTC) و مهار مالون دی آلدئید با روش تیوباربیوتریک اسید (TBA) در مقابل استانداردهای بوتیلیت هیدروکسی تولوئن (BHT)، بوتیلیت هیدروکسی آنیسول (BHA) و آسکوربیک اسید مورد بررسی قرار گرفت. نتایج و بحث: محتوای فنلی در ریشه (58/2±61/61 میکروگرم معادل گالیک اسید در هر میلی گرم وزن خشک عصاره) طبق روش فولین-سیوکالتیو بیشتر از سایر اندامها بود. در مهار نیمی از رادیکال های آزاد DPPH عصاره ریشه، بذر و برگ به ترتیب با 02/2±9/238 ، 42/1±78/253 و 56/2±95/277 میکرو گرم در میلی لیتر بهترین عملکرد را داشتند. در مهار پراکسیداسیون لینولئیک اسید، عصاره ریشه بهترین قدرت مهاری را بعد از آسکوربیک اسید و BHT داشت و عصاره های برگ و دانه عملکردی مشابه با BHA داشتند. در مهار مالون دی آلدئید عصاره ریشه و دانه به ترتیب با 54/0±68/78 و 66/0±44/77 درصد نسیت به سایر عصاره ها بهترین عملکرد را داشتند. ضریب تشخیص پیرسون بین محتوای فنلی و ظرفیت آنتی اکسیدانی عصاره ها مطابق روش های DPPH، FTC و TBAبالا و معادل 838/0-، 895/0 و 740/0 بود. توصیه کاربردی/ صنعتی: نتایج این تحقیق می تواند نوید بخش استفاده از توان آنتی اکسیدانی اندام این گیاه بویژه در مهار رادیکال های آزاد و پراکسیداسیون لیپیدی باشد.
Keywords [Persian]
Catharanthus roseus
پراکسیداسیون چربی
مالون دی آلدهید
دفاع آنتی اکسیدانی غیر آنزیمی
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