Effects of Jasmonic acid on essential oil yield and chemical compositions of two Iranian landraces of basil (Ocimum basilicum) under reduced irrigation

Document Type: Original article

Authors

1 Department of Plant Biology, Faculty of Biology Science, Kharazmi University, 15719-14911, Tehran, Iran

2 Medicinal Plants Department, Shahrekord Branch, Islamic Azad University, Shahrekord, 88146, Iran; Medicinal Plants Program, College of Natural Sciences, Massachusetts University, Amherst, 01003, MA, USA

Abstract

Background & Aim: Sweet basil (Ocimum basilicum), a plant that is extensively cultivated in some countries, is used to enhance the flavour of salads, sauces, pasta, confectioneries and other products as both a fresh and dried herb. The effect of foliar application of Jasmonic acid and reduced irrigation on essential oil yield and chemical components of two Iranian landrace of basil (Ocimum basilicum) were investigated. Experimental: Treatments comprised control, 0.0, 200 and 400 µL Jasmonic acid applied to plants under normal irrigation and stressed conditions (30 and 60% F.C) based on a completely randomized design with three replications. The essential oils from the aerial parts of basil were analyzed by GC-FID and GC/MS. Results & Discussion: Results indicated that the different levels of Jasmonic acid and irrigation had significant effects on oil yield and some main components of the essential oil. The highest value of oil content was obtained from application of 400 µL JA. Percentage of some chemical constituents in the essential oil extracted from the plants under stress was higher than non-stressed plants. Foliar application of Jasmonic acid significantly improved methyl chavicol in the oils, but reduced Germacrene- D, α-Cadinol and δ-Cadinene amounts. Industrial and practical recommendations: Since, essential oil of basil, particularly methyl chavicol component, have many application in pharmaceutical and perfumery industry and has health benefits such as antiviral, antibacterial and antispasmodic activity, we can use Jasmonic acid elicitor to enhance the therapeutic properties of basil and also essential oil content and methyl chavicol of plant.

Keywords

Article Title [Persian]

اثر اسید جاسمونیک بر راندمان و ترکیب شیمیایی اسانس دو نژاد بومی ریحان ایرانی (Ocimum basilicum) تحت تنش خشکی

Authors [Persian]

  • فاطمه ملک پور 1
  • اعظم سلیمی 1
  • عبدالله قاسمی پیربلوطی 2
1 گروه زیست شناسی، دانشکده علوم زیستی، دانشگاه خوارزمی، تهران، ایران
2 گروه گیاهان دارویی، دانشگاه آزاد اسلامی شهرکرد، شهرکرد، ایران گروه علوم گیاهی ، کالج علوم طبیعی، دانشگاه ماساچوست، ماساچوست، آمریکا
Abstract [Persian]

مقدمه و هدف: ریحان Ocimum basilicum یک گیاه دارویی است که به طور وسیعی در بعضی کشورها کشت می شود  و به صورت تازه و خشک شده برای افزایش عطر و طعم سالاد، سس، ماکارونی، شیرینی و سایر محصولات به کار می­ رود. در این پژوهش، اثر محلول پاشی اسیدجاسمونیک و کمبود آبیاری بر عملکرد و ترکیبات اسانس دو نژاد بومی ریحان ایرانی مورد بررسی قرار گرفت. روش تحقیق:تیمارها شامل اسیدجاسمونیک (کنترل، 200 و 400 میکرولیتر) و تنش خشکی (بدون تنش، 30 و 60 درصد ظرفیت زراعی) بود که بر اساس یک طرح کاملاً تصادفی با سه تکرار انجام شد. ترکیبات اسانس حاصل از قسمت های هوایی  توسط   GC-FID و   GC/MSشناسایی گردید. نتایج و بحث: نتایج نشان داد که سطوح مختلف اسید جاسمونیک و آبیاری اثر معنی داری بر عملکرد و برخی از اجزای اصلی اسانس داشت. بالاترین میزان اسانس  با تیمار 400 میکرولیتر اسید جاسمونیک به دست آمد. درصد برخی از ترکیبات شیمیایی اسانس گیاهان، تحت تنش خشکی بالاتر از گیاهان بدون تنش بود. محلول پاشی اسید جاسمونیک به طور قابل توجهی میزان ترکیب متیلچاویکول را افزایش داد، اما میزان ترکیبات جرماکرنD، آلفا-کادینول، دلتا کادینن را کاهش داد. توصیه کاربردی/ صنعتی:از آنجایی که اسانس ریحان به ویژه ترکیب متیل چاویکول در صنعت داروسازی و عطرسازی کاربرد دارد و دارای خواص درمانی نظیر خاصیت ضدویروسی، ضدباکتریایی و ضداسپاسم می باشد، می توان از الیسیتور اسیدجاسمونیک برای افزایش خصوصیات درمانی ریحان بهره جست و میزان اسانس و ترکیب متیل چاویکول را در گیاه افزایش داد.

Keywords [Persian]

  • ریحان
  • اسیدجاسمونیک
  • متابولیت های ثانویه
  • تنش خشکی

References

Adams, R.P. 2007. Identification of essential oil components by gas chromatog-raphy/quadrupole mass spectroscopy. Allured Publishing Corporation, CarolStream, IL, USA.

Bettaieb, I., Zakhama, N., Wannes, W.A., Kchouk, M. E., Marzouk, B. 2009. Water deficit effects on Salvia officinalis fatty acids and essential oils composition. Scientia Horticulturae, 120: 271–275.

Boonlertnirun, S., Boonraung, C., Suvanasara, R. 2008. Application of chitosan in Rice production. International Journal of Minerals, Metallurgy, and Materials, 18:47–52.

British Pharmacopoeia, 1988. British Pharmacopoeia, vol. 2. HMSO, London, pp.137–138.

Chiang, L.C., Ng, L.T., Cheng, P.W., Chiang, W., Lin, C.C. 2005. Antiviral activities of extracts and selected pure constituents of Ocimum basilicum. Clinical and Experimental Pharmacology and Physiology, 32(10): 811–816.

Deshpande, R.S., Tipnis, H.P. 1997. Insecticidal activity of Ocimum basilicum L. Pesticides, 11, 1–12.

Ghasemi Pirbalouti, A., Samani, M.R., Hashemi, M., Zeinali, H. 2013. Salicylic acid affects growth, essential oil and chemical compositions of thyme (Thymus daenensis Celak.) under reduced irrigation. Plant Growth Regulation, 72: 289–301.

Ghasemi Pirbalouti, A. 2014. Diversity in chemical composition and yield of essential oil from two Iranian landraces of sweet basil. Genetika, 46: 419–426.

Ghasemi Pirbalouti, A., Sajjadi, S.E., Parang, K. 2014. A review (research and patents) on jasmonic acid and its derivatives. Archiv der Pharmazie, 347: 229–239.

Ghasemi Pirbalouti, A., Ghahfarokhi, B., Ghahfarokhi, S.A.M., Malekpoor, F. 2015. Chemical composition of essential oils from the aerial parts and underground parts of Iranian valerian collected from different natural habitats. Industrial Crops and Products, 63: 147–151.

Halitschke, R., Kessler, A., Kahl, J., Lornez, A., Baldwin, I.T. 2000. Ecophysiological comparison of direct and indirect defenses in Nicotiana attenuata. Oecologia, 124: 408-417.

Hussain, A.I., Anwar, F., Hussain Sherazi, S.T., Przybylski, R. 2008. Chemical composition, antioxidant and antimicrobial activities of basil (Ocimum basilicum) essential oils depends on seasonal variations. Food Chemistry, 108(3): 986–995.

Hussain, S., Fareed, S., Ansari, S., Rahman, A., Ahmad, I.Z., Saeed, M. 2012. Current approaches toward production of secondary plant metabolites. Journal of Pharmacy and Bioallied Sciences, 4: 10–20.

IRIMO, 2012. (Islamic Republic of Iran Meteorological Organization). Climatology methods. http://www.irimo.ir.

Keina, M., Oldham, N., Baldwin, I.T. 2001. Rapid HPLC screening of jasmonate-induced increases in tobacco alkaloids, phenolics, and diterpene glycosides in Nicotiana attenuate. Journal of Agricultural and Food Chemistry, 49: 3553-3558.

Khalid, K.A. 2006. Influence of water stress on growth, essential oil and chemical composition of herbs (Ocimum sp.). International Agrophysics, 20: 289–296.

Kwee, E.M., Niemeyer, E.D. 2011. Variations in phenolic composition and antioxidant properties among 15 basil (Ocimum basilicum L.) cultivars. Food Chemistry, 128(4): 1044–1050.

Martin, D., Tholl, D., Gershenzon, J., Bohlmann, J. 2002. Methyl jasmonate induces traumatic resin ducts, terpenoid resin biosysthesis, and terpenoid accumulation in developing xylem of Norway spruce stems. Plant Physiology, 129: 1003-1018.

Rabea, E.I., Badawy, M.E., Stevens, C., Smagghe, G., Steurbaut, W. 2003. Chitosan as antimicrobial agent: applications and mode of action. Biomacromolecules, 4: 1457-1465.

Sajjadi, S.E. 2006. Analysis of the essential oils of two cultivated basil (Ocimum basilicum L.) From Iran. DARU Journal of Pharmaceutical Sciences, 14: 128–130.

Shokrani, F., Pirzad, A., Zardoshti, M., Darvishzadeh, R. 2012. Effect of biological nitrogen on the yield of dried flower and essential oil of Calendula officinalis L. under end season water deficit condition. International Research Journal of Applied and Basic Sciences, 3: 24–34.

Simon, J.E., Reiss-Bubenheim, D., Joly, R.J., Charles, D.J. 1992. Water stress-induced alterations in essential oil content and composition of sweet basil. Journal of Essential Oil Research, 4: 71–75.

Simon, J.E., Chadwick, A.F., Craker, L.E. 1984. Herbs: an indexed bibliography, 1971-1980. The scientific literature on selected herbs, and aromatic and medicinal plants of the temperate zone. Archon Books: Hamden, CT.

Turtola, S., Manninen, A.M., Rikala, R., Kainulainen, P. 2003. Drought stress alters the concentration of wood terpenoids in Scots pine and Norway spruce seedlings. Journal of Chemical Ecology, 29:1981–1985.

Wasternack, C., Parthier, B. 1997. Jasmonate-signaled plant gene expression. Trends Plant Science, 2: 1360-1385.

Zhao, J., Davis, L.C., Verpoorte, R. 2005. Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnology Advances, 23: 283–333.

Journal of Herbal Drugs (An International Journal on Medicinal Herbs)

Volume 6, Issue 1
Spring 2015
Pages 13-21

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