The effects of Thyme extrac (Thymus vulgaris) on systemic antibody responses against Influenza and Newcastle disease vaccine in broiler chickens

Document Type: Original article

Authors

1 Department of Clinical Sciences, Faculty of Veterinary Medicine Shahid Chamran University of Ahvaz- Iran

2 Department of Basic Sciences, Faculty of Veterinary Medicine Shahid Chamran University of Ahvaz- Iran

Abstract

Background & Aim: The recent study was conducted to investigate the effects of Thyme extract in drinking water on immune response of broiler chickens. Experimental:  A total of 245 day-old broiler chicks were purchased and 20 chicks were bled for determination maternal antibody and remaining chicks divided into 5 equal groups. Chickens of group A, B and C received 0.1%, 0.15% and 0.2% ofThyme extract in drinking water for all of the period of experiment, respectively. Chickens of group D did not receive Thyme extract but vaccinated against Newcastle and Influenza diseases. Chickens of group E were kept as control group and did not receive Thyme extract and Newcastle and Influenza diseases vaccines. Chickens of group A, B, C and D were vaccinated with live Newcastle vaccine (B1 strain) intraocularly and AI-ND killed vaccine (subtype H9N2) subcutaneously of neck back. Blood samples were collected before vaccination as well as on days 14, 28 and 35 after vaccination. Ten chickens of each group were bled randomly and antibody titer against Newcastle and Influenza vaccine virus was determined by HI test. Results & Discussion: The results of present study showed that Thyme extract has no effect on antibody response against Newcastle vaccine virus, but 14 days after vaccination, receiving of 0.2% ofThyme extract, significantly increased the specific antibody response against Influenza vaccine virus compared to all groups. Industrial and practical recommendations: Herbs that are rich in flavonoids such as thyme (Thymus vulgaris) extend the activity of vitamin C, act as antioxidants and may therefore enhance the immune function.

Keywords


Article Title [Persian]

اثرعصاره ی آویشن (Thymus vulgaris) بر پاسخ ایمنی در برابر ویروس واکسن بیماری آنفلوانزا و نیوکاسل در جوجه‌های گوشتی

Authors [Persian]

  • فروغ طلازاده 1
  • منصور میاحی 1
  • حسین نجف زاده ورزی 2
  • حدیث نوروزی زاده 2
1 گروه علوم بالینی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، ایران
2 گروه علوم پایه، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، ایران
Abstract [Persian]

مقدمه و هدف:مطالعه حاضر به منظور بررسی تاثیر عصاره آویشن بر پاسخ ایمنی علیه واکسن بیماری آنفلوانزا و نیوکاسل انجام شد. روش تحقیق: دویست و چهل و پنج قطعه جوجه‌ی یک روزه‌ی گوشتی خریداری و  بیست قطعه جوجه برای تعیین میزان پادتن مادر‌ی خون‌گیری شده و بقیه­ی جوجه­ها به طور تصادفی به 5 گروه مساوی تقسیم شدند. جوجه­های گروه A،B وC عصاره­ی آبی آویشن را به ترتیب به میزان 1/0 درصد، 15/0 درصد و 2/0 درصد در آب آشامیدنی در طول دوره­ی پرورش دریافت نمودند. جوجه­های گروه E، عصاره­ی آویشن را دریافت نکردند اما علیه بیماری نیوکاسل واکسینه شدند. جوجه­های گروه F به عنوان گروه کنترل، عصاره­ی آویشن و واکسن نیوکاسل دریافت نکردند. جوجه­های گروه­های A، B، C و D، با واکسن زنده‌ی B1 به روش قطره­­ی چشمی و واکسن کشته‌ی دوگانه نیوکاسل-آنفلوانزای تحت ‌تیپ (H9N2)، به روش زیر پوست پشت گردن واکسینه شدند. در روزهای صفر (قبل از واکسیناسیون)، 14، 28 و 35 بعد از واکسیناسیون، از 10 قطعه جوجه از هر گروه، به طور تصادفی، خون‌گیری به عمل آمد و عیار پادتن ویژه­ی نیوکاسل به وسیله آزمایش ممانعت از هماگلوتیناسیون تعیین گردید. نتایج و بحث:نتایج این مطالعه نشان داد که عصاره­ی آویشن، تاثیری برپاسخ پادتن ویژه­ی ویروس واکسن نیوکاسل ندارد اما دریافت 2/0 درصد عصاره آویشن در 14 روز بعد از واکسیناسیون، عیار پادتن ویژه­ی ویروس واکسن آنفلوانزا را در مقایسه با سایر گروه ها به طور معنی داری افزایش داد. توصیه کاربردی/ صنعتی: گیاهانی مانند آویشن که سرشار از فلاونوئیدهامی باشد فعالیت ویتامین C را توسعه می دهند، به عنوان آنتی اکسیدان عمل می کنند و بنابراین ممکن است عملکرد سیستم ایمنی بدن را افزایش دهند.

Keywords [Persian]

  • عصاره ی آویشن
  • پاسخ ایمنی
  • بیماری نیوکاسل
  • جوجه ی گوشتی
  • بیماری آنفلوانزا
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