Adel, M., Abedian Amiri, A., Divband, M., Safari, R., Khalili, E. (2015). Chemical composition and antimicrobial activity of Achillea wilhelmsii C.Koch essential oil against selected bacterial and fungal pathogens of fish. Journal of Herbal Drugs
(An International Journal on Medicinal Herbs), 6(2), 65-71.
Milad Adel; Armin Abedian Amiri; Maryam Divband; Reza Safari; Elham Khalili. "Chemical composition and antimicrobial activity of Achillea wilhelmsii C.Koch essential oil against selected bacterial and fungal pathogens of fish". Journal of Herbal Drugs
(An International Journal on Medicinal Herbs), 6, 2, 2015, 65-71.
Adel, M., Abedian Amiri, A., Divband, M., Safari, R., Khalili, E. (2015). 'Chemical composition and antimicrobial activity of Achillea wilhelmsii C.Koch essential oil against selected bacterial and fungal pathogens of fish', Journal of Herbal Drugs
(An International Journal on Medicinal Herbs), 6(2), pp. 65-71.
Adel, M., Abedian Amiri, A., Divband, M., Safari, R., Khalili, E. Chemical composition and antimicrobial activity of Achillea wilhelmsii C.Koch essential oil against selected bacterial and fungal pathogens of fish. Journal of Herbal Drugs
(An International Journal on Medicinal Herbs), 2015; 6(2): 65-71.
Chemical composition and antimicrobial activity of Achillea wilhelmsii C.Koch essential oil against selected bacterial and fungal pathogens of fish
1Department of Aquatic Animal Health and Diseases, Iranian Fisheries Research Center, Tehran, Iran
2Department of Aquatic Animal Health and Diseases, Caspian Sea Ecology Research Center, Sari, Iran
3Department of Food and Drug, Zahedan University of Medical Sciences, Zahedan, Iran
4Department of Health and Food Quality Control, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran
Abstract
Background & Aim: By increasing resistance of fish pathogens to chemical drugs, using herbal plants with antimicrobial properties is necessary.In this study, the composition of the essential oil and antimicrobial activity of aerial parts of Achillea wilhelmsii C.Koch growing wild in Zagross mountain (Southwest of Iran) was analyzed against common bacterial and fungal pathogens of fish. Experimental: In the present study, aerial parts of A. Wilhelmsii were tested against five bacterial strains including Streptococcus iniae, Yersinia ruckeri, Vibrio angularum, Aeromonas hydrophila and Pseudomonas aeruginosa and four pathogenic fungi namely Saprolegnia sp., Fusarium solani, Candida albicansand Aspergillus flavus. Also, the oil constituents of A. Wilhelmsii were analyzed by gas chromatography/mass spectrometry (GC/MS). Results & Discussion: The major composition of the essential oil of A. Wilhelmsii was 1.8-cineol (25.2%), Camphor (18.9%), Linalool (6.9%), Borneol (5.7%) and Artemisia alcohol (4.3). All microorganisms showed more sensitivity to essential oil of A. Wilhelmsii than the control antibiotics. Maximum antibacterial and antifungal activity was observed against Y. ruckeri (25.4 mm)and C. albicans (25 mm) respectively, while S. iniae (16.4 mm)and A. flavus (14 mm)exhibited the least sensitivity. In addition, MIC test showed that minimum concentrations of the essential oil ranged from 125 to 800 μg/l were able to inhibit the growth of the selected bacterial and fungal pathogens. Industrial and practical recommendations: The results showed that the essential oil of A. Wilhelmsii could be a potential source of new and effective antibacterial components in aquaculture industry. Although, more study needed to purify, fractional and characterize various antimicrobial compounds from the essential oil of A. Wilhelmsii.
1Department of Aquatic Animal Health and Diseases, Iranian Fisheries Research Center, Tehran, Iran
2Department of Aquatic Animal Health and Diseases, Caspian Sea Ecology Research Center, Sari, Iran
3Department of Food and Drug, Zahedan University of Medical Sciences, Zahedan, Iran
4Department of Health and Food Quality Control, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran
Abstract [Persian]
مقدمه و هدف: با افزایش مقاومت عوامل بیماریزا در ماهی نسبت به داورهای شیمیایی، استفاده از گیاهان دارویی با خواص ضد میکروبی ضروری به نظر میرسد. در این مطالعه ترکیبات شیمیایی بخشهای هوایی اسانس بومادران وحشی پرورش یافته در رشتهکوههای زاگرس (جنوب غربی ایران) تعیین و خواص ضد میکروبی آن بر روی باکتریها و قارچهای بیماریزای شایع در ماهی مورد بررسی قرار گرفت. روش تحقیق: در این مطالعه، خواص ضد میکروبی اسانس بومادران علیه پنج باکتری شامل استرپتوکوکوس اینیائی، یرسینیا راکری، ویبریو آنگوئیلاروم، آئروموناس هیدروفیلا و سودوموناس آئروجینوزا و چهار قارچ بیماریزای: ساپرولگنیا، فوزاریوم سولانی، کاندیدا آلبیکنز و آسپرژیلوس فلاوس مورد ارزیابی قرار گرفت. همچنین، ترکیبات تشکیل دهنده اسانس بومادران با استفاده از دستگاه گاز کروماتوگرافی- طیف سنج جرمی (GC/MS) تعیین شد. نتایج و بحث: بازده اسانس جمع آوری شده از استان چهارمحال و بختیاری 63/0% بود. مهمترین ترکیبات تشکیل دهنده اسانس بومادران شامل: 8-1 سینئول (2/25%)، کامفور (9/18%)، لینالول (9/6%)، بارنئول (7/5%) و آرتیمزیا (3/4%) بود. همه میکروارگانیسمهای مورد مطالعه نسبت به اسانس بومادران حساسیت بیشتری را نسبت به آنتی بیوتیکهای کنترل نشان دادند. بشترین خواص ضد باکتری و ضدقارچی بومادران به ترتیب علیه باکتری یرسینیا راکری (با قطر هاله مهار رشد 4/25 میلیمتر) و قارچ کاندیدا آلبیکنز (25 میلیمتر) مشاهده شد. این در حالی است که کمترین حساسیت نسبت به باکتری استرپتوکوکوس اینیائی (4/16 میلیمتر) و قارچ آسپرژیلوس فلاوس (14 میلیمتر) مشاهده شد. علاوه بر این، حداقل غلظت مهار کنندگی رشد (MIC) اسانس مورد مطالعه در محدوده 125 تا 800 میکروگرم/ لیتر تعیین شد که قادر به مهار رشد باکتریها و قارچهای بیماریزای منتخب بود. توصیه کاربردی/صنعتی: نتایج این تحقیق نشان دهنده آن است که اسانس بومادران میتواند به عنوان یک منبع بالقوه جدید و موثرتر از آنتی بیوتیکهای تجاری در صنعت آبزیان مورد استفاده قرار گیرد. هر چند که، انجام مطالعات بیشتر به منظور خالص سازی، جداسازی و تعیین ترکیبات دارای خواص ضد میکروبی اسانس بومادران ضروری به نظر میرسد.
Keywords [Persian]
بومادران، کوه زاگرس، ترکیبات تشکیل دهنده اسانس، فعالیت ضد میکروبی
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