The effects of hydro-alcohol extract of follower of marshmallow (Althaea officinalis L.) on some biochemical and hematological parameters in common carp (Cyprinus carpio L.)

Document Type: Original article

Authors

1 Department of Aquatic production, College of Natural Resource, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran

2 Assistant professor, Aquaculture Department, Natural Resources and Environmental Faculty, Behbahan Khatam Alanbia University of Technology, Iran

Abstract

Background & Aim: Marshmallow (Althaea officinalis) is one of the most popular medicinal plants used in traditional medicine with antibacterial and antioxidant properties. This study was conducted to evaluate the effects of marshmallow extract (Althaea officinalis L.) administration on blood cells and biochemical parameters of carp liver. Experimental: 150 carp (weighing 37.65 ± 4.40 g) were fed with diets containing 0.0 (control diet), 2.5, 5, and 10 g marshmallow extract for 60 days. Then, red blood cell indices (RBC), hemoglobin (Hb), hematocrit (Hct), mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCH), white blood cells differential count and biochemical parameters of liver such as aspartate aminotransferase (AST), alanine amino transferase (ALT), lactate dehydrogenase (LDH), and alkaline phosphatase (ALP) were measured on days 30 and 60 of the experiment. Results & Discussion: On days 30 and 60 of the experiment, red blood cell indices (RBC), hemoglobin (Hb), hematocrit (Hct), mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCH), white blood cells differential count and biochemical parameters of liver such as aspartate aminotransferase (AST), alanine amino transferase (ALT), lactate dehydrogenase (LDH), and alkaline phosphatase (ALP) were measured. On day 60, no significant difference was found (p>0.05) in RBC, Hb, Htc, MCV, MCH count, number of neutrophils, basophils, and eosophils in fish fed with different concentrations of marshmallow extract. Administration of marshmallow extract (5 g) significantly increased (p<0.05) WBC and lymphocytes on day 60. On the other hand, administration with 5 and 10 g of marshmallow extract decreased monocytes (p<0.05) on day 30. The significant increase was observed in AST, ALT, ALP and LDH levels in liver of fish fed with 10 g of marshmallow extract may be attributed to cytotoxicity, however 2.5 and 5 g of extract did not have adverse effects on liver enzymes. Industrial and practical recommendations: According to the results, it was concluded that preclinical administration of certain concentrations of marshmallow extract (2.5 and 5 g) was beneficial for carp.

Keywords


Article Title [Persian]

تاثیرات عصاره هیدروالکلی گل ختمی (Althaea officinalis L) بر برخی پارامترهای بیوشیمیایی و خونی ماهی کپور معمولی (Cyprinus carpio

Authors [Persian]

  • فهیمه فلاح پور 1
  • مهدی بنایی 2
  • نرگس جوادزاده 1
1 گروه شیلات، پردیس علوم و تحقیقات خوزستان، دانشگاه آزاد اسلامی، اهواز، ایران
2 گروه شیلات، دانشکده منابع طبیعی و محیط زیست، دانشگاه صنعتی خاتم الانبیاء (ص) بهبهان، بهبهان، ایران
Abstract [Persian]

مقدمه و هدف: گیاه ختمی (Althae officinalis L.) یکی از معروف­ترین گیاهان دارویی است که به دلیل داشتن خواص ضد باکتریایی و آنتی­اکسیدانی در طب سنتی مورد استفاده قرار می­گیرد. هدف از انجام این مطالعه، بررسی تاثیر تجویز عصاره گل ختمی بر پارامترهای خونی و بیوشیمیایی در ماهی کپور است. روش تحقیق:در این آزمایش 150 عدد ماهی کپور معمولی (با میانگین وزنی 40/4±65/37 ) با جیره­های حاوی 0/0 (کنترل)، 5/2، 5 و 10 گرم عصاره گل ختمی به مدت 60 روز تغذیه شدند. سپس در روزهای 30 و 60 آزمایش، شاخص­های خون­شناسی و بیوشیمیایی ماهی­ها اندازه­گیری شد. نتایج و بحث: هیچ گونه تغییر معنی­داری در تعداد سلول قرمز خون، هموگلوبین، هماتوکریت و میانگین حجم گویچه­ها، تعداد نوتروفیل­ها، بازوفیل­ها و ائوزینوفیل­ها در ماهیانی که با غلظت­های مختلف عصاره گل ختمی به مدت 60 روز تغذیه شدند، مشاهده نگردید. تجویز عصاره ختمی (5 گرم) موجب افزایش معنی­داری در تعداد سلول­های سفید خون و لنفوسیت­ها در روز 60 شد. از سویی دیگر تجویز 5 و 10 گرم عصاره ختمی سبب کاهش معنی­دار منوسیت­ها گردید. تجویز عصاره ختمی در طی 60 روز تاثیر معنی­داری بر فعالیت آنزیم­های آلانین آمینوترانسفراز، آلکالین فسفاتاز و کراتین­فسفوکیناز و نیز سطح کراتینین خون نداشت. فعالیت آنزیم آسپارتات آیمنوترانسفراز و لاکتات دهیدروژناز پلاسما و همچنین سطح پروتئین کل پلاسما و آلبومین به طور معنی­داری در ماهیان تحت تیمار غلظت 10 گرم عصاره ختمی در بالاترین سطح قرار داشت. سح کلسترول و تری­گلیسرید نیز در گروه­های تحت تیمار غلظت­های 5 و 10 گرم عصاره ختمی به طور معنی­داری کاهش یافت. تغییرات معنی­داری مشاهده شده در فاکتورهای خونی و بیوشیمیایی ماهیان تحت تیمار غلظت 10 گرم عصاره ختمی ممکن است به سمیت سلولی نسبت داد؛ در حالی که تجویز 5/2 و 5 گرم عصاره ختمی تاثیر سوئی بر شاخص­های خونی و بیوشیمیایی ماهیان کپور در این مطالعه نداشت. توصیه کاربردی/ صنعتی:براساس این نتایج، تجویز پیش­بالینی غلظت­های مشخصی از عصاره گل ختمی (5/2 و 5 گرم) برای ماهیان کپور معمولی مفید بوده است.

Keywords [Persian]

  • کپور معمولی
  • عصاره گل ختمی
  • سلول‌های خونی
  • فاکتورهای بیوشیمیایی خون
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