Khodabande, M., Rouhi, L., Ghasemi Pirbaluti, A. (2015). Dose-dependent effects of Gundelia Tournefortii root extract on meiosis resumption and in vitro maturation in mouse immature oocytes. Journal of Herbal Drugs
(An International Journal on Medicinal Herbs), 6(3 Special issue (in Persian or Farsi)), 161-166.
Marzieh Khodabande; Leila Rouhi; Abdollah Ghasemi Pirbaluti. "Dose-dependent effects of Gundelia Tournefortii root extract on meiosis resumption and in vitro maturation in mouse immature oocytes". Journal of Herbal Drugs
(An International Journal on Medicinal Herbs), 6, 3 Special issue (in Persian or Farsi), 2015, 161-166.
Khodabande, M., Rouhi, L., Ghasemi Pirbaluti, A. (2015). 'Dose-dependent effects of Gundelia Tournefortii root extract on meiosis resumption and in vitro maturation in mouse immature oocytes', Journal of Herbal Drugs
(An International Journal on Medicinal Herbs), 6(3 Special issue (in Persian or Farsi)), pp. 161-166.
Khodabande, M., Rouhi, L., Ghasemi Pirbaluti, A. Dose-dependent effects of Gundelia Tournefortii root extract on meiosis resumption and in vitro maturation in mouse immature oocytes. Journal of Herbal Drugs
(An International Journal on Medicinal Herbs), 2015; 6(3 Special issue (in Persian or Farsi)): 161-166.
Dose-dependent effects of Gundelia Tournefortii root extract on meiosis resumption and in vitro maturation in mouse immature oocytes
2Medicinal Plants Department, Shahrekord Branch, Islamic Azad University, Shahrekord
Abstract
Background & Aim: Oocytes in vitro maturation is an enormously promising technology for the treatment of infertility, yet its clinical application remains limited owing to poor success rates. Therefore this study was designed to determine the effect of Gundelia Tournefortii root hydro alcoholic extract as a source of antioxidant on in vitro oocyte maturation. Experimental: In this study, the immature oocytes nucleated were obtained from NMRI mice (6-8 weeks). Germinal vesicles (GV) were cultured for 24 hours in maturation medium in MEMα supplemented with 7.5IU/ml hCG, 100mIU/ml rhFSH, 5% FCS (control group) and adding 10µg/ml Gundelia Tournefortii extract (group 1), 20µg/ml Gundelia Tournefortii extract (group 2) and 40µg/ml Gundelia Tournefortii extract (group 3). Then in vitro maturation stages and resumption of meiotic in all groups was recorded by inverted microscope. Results & Discussion: In group 1 that the oocytes were exposed 10µg/ml of Gundelia Tournefortiiroot extract,maturation rates was significantly higher compared with the control group (p<0/05). In group 2, the oocytes were exposed 20µg/ml of Gundelia Tournefortiirootextract, maturation rates showed a significant increase compared to the control group (p<0/05). In group 3 the oocytes were exposed 40 µg/ml of Gundelia Tournefortiirootextract, also maturation rates showed a significant increase compared to the control group (p<0/05). Industrial and practical recommendations: The results of this study showed that the Gundelia Tournefortiirootextract, has a positive doses dependent effect on oocyte maturation. So with increasing concentration of Gundelia Tournefortiirootextract, the rate of maturation immature oocytes is increased.
1گروه فیزیولوژی جانوری، دانشکده علو م پایه، دانشگاه آزاد اسلامی واحد شهرکرد، شهرکرد، ایران
2گروه گیاهان دارویی، دانشکده کشاورزی، دانشگاه آزاد اسلامی واحد شهرکرد، شهرکرد، ایران
Abstract [Persian]
مقدمه و هدف: بلوغ آزمایشگاهی تخمک یک روش مناسب در جهت درمان ناباروری می باشد، که استفاده کلینیکی آن به واسطه موفقیت پایین با محدودیت مواجه می باشد. این مطالعه به منظور بررسی اثر عصاره آبی ریشه کنگر، به عنوان یک منبع آنتی اکسیدانی، بر میزان بلوغ تخمکها در شرایط آزمایشگاهی طراحی شد. روش تحقیق: در این مطالعه آزمایشگاهی، تخمک های نارس از تخمدان موش های سوری نژاد NMRI (4-6 هفتهای) جمع آوری شدند. تخمکهای نارس به مدت 24 ساعت در محیط کشتMEM-α کامل شده با 5% FCS، ml /IUhCG 5/7، mIUrFSH ١٠٠ کشت داده شدند (گروه کنترل) و غلظت 10 میکروگرم/میلیلیتر عصاره (گروه 1)، 20 میکروگرم/میلیلیتر عصاره (گروه 2) و 40 میکروگرم/میلیلیتر عصاره (گروه 3) به محیط کشت بلوغ اضافه گردید. با استفاده از میکروسکوپ اینورت مراحل بلوغ آزمایشگاهی و از سر گیری میوز در تمام گروه ها بررسی شد. نتایج و بحث: در گروه آزمایشی 1 که تخمک ها در معرض 10 میکروگرم/میلیلیتر از ریشه برگ کنگر بودند، میزان بلوغ نسبت به گروه کنترل افزایش معنی داری را نشان دادند. در گروه آزمایشی 2 که تخمک ها در معرض 20 میکروگرم/میلیلیتر از عصاره بودند، میزان بلوغ نسبت به گروه کنترل افزایش معنی داری را نشان می داد (p<0/05 ). در گروه آزمایشی 3 که تخمک ها در معرض 40 میکروگرم/میلیلیتر از عصاره ریشه کنگر بودند نیز میزان بلوغ نسبت به گروه کنترل افزایش معنیداری را نشان داد.
توصیه کاربردی/ صنعتی: نتایج این تحقیق نشان داد که تاثیر عصاره آبی ریشه کنگر، دارای یک اثر مثبت وابسته به دوز در بلوغ تخمک می باشد، بطوریکه با افزایش میزان غلظت عصاره، میزان بلوغ تخمک های نارس نیز افزایش می یابد.
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