Saghaei, F., Salehi, M., Namzi, M., Bagheri, M. (2015). Gastroprotective effects of Thymus daenensis hydroalcoholic extract against ethanol-induced gastric mucosal ulcers in rats. Journal of Herbal Drugs
(An International Journal on Medicinal Herbs), 6(3 Special issue (in Persian or Farsi)), 167-174.
Firuzeh Saghaei; Mohammad Salehi; Mohammad Javad Namzi; Mohammad Bagheri. "Gastroprotective effects of Thymus daenensis hydroalcoholic extract against ethanol-induced gastric mucosal ulcers in rats". Journal of Herbal Drugs
(An International Journal on Medicinal Herbs), 6, 3 Special issue (in Persian or Farsi), 2015, 167-174.
Saghaei, F., Salehi, M., Namzi, M., Bagheri, M. (2015). 'Gastroprotective effects of Thymus daenensis hydroalcoholic extract against ethanol-induced gastric mucosal ulcers in rats', Journal of Herbal Drugs
(An International Journal on Medicinal Herbs), 6(3 Special issue (in Persian or Farsi)), pp. 167-174.
Saghaei, F., Salehi, M., Namzi, M., Bagheri, M. Gastroprotective effects of Thymus daenensis hydroalcoholic extract against ethanol-induced gastric mucosal ulcers in rats. Journal of Herbal Drugs
(An International Journal on Medicinal Herbs), 2015; 6(3 Special issue (in Persian or Farsi)): 167-174.
Gastroprotective effects of Thymus daenensis hydroalcoholic extract against ethanol-induced gastric mucosal ulcers in rats
Department of Pharmacology, Faculty of Veterinary Medicine , Islamic Azad University , Shahrekord branch, Shahrekord , Iran
Abstract
Background & Aim: Ulcer index is one of the most common diseases among people. Current therapies include acid secretion inhibitors which have various side effects. Herbal medicines are considered as adjuvant therapy for this disease. Hydroalcoholic extract of Thymusdaenensis has various components such as flavonoids which contribute to gastroprotection. Experimental: 35 Sprague Dawley rats (200-250 g weight) were randomly divided into 7 groups: Group 1 received salin, Groups 2 and 3 and 4 (experimental groups) received ethanolic extract of Thymusdaenensis (500 and 1000 and 1500 mg/kg respectively), Group 5 received omeprazole 20 mg/kg., Group 6 received orally carboxy methyl cellulose (omeprazol vehicle), Group 7(negative control) received saline. One hour later absolute ethanol was given orally to all rats except group 7 to induce gastric mucosal injury. After 4 hrs. the rats were anesthetized and sacrificed. Blood samples were collected through heart and were administrated to SOD measurement and gastric tissues were removed for histopathologic study and MDA assessment. Results & Discussion: The extract of plant was a significant and dose dependent effect to reduce ulcer index and to increase the percentage of ulcer inhibition. The study also indicated a significant increase in SOD activity in serum, whereas a significant decrease in MDA was observed. The ulcer protective effect of this plant may possibly be due to antioxidant effects and reduction of oxidative stress.
اثر عصاره هیدروالکلی آویشن دنایی بر پیشگیری از زخم معده القا شده با اتانول در رت
Authors [Persian]
فیروزه سقایی; محمد صالحی; محمد جواد نمازی; محمد باقری
دانشکده دامپزشکی دانشگاه آزاد اسلامی واحد شهرکرد، شهرکرد، ایران
Abstract [Persian]
مقدمه و هدف: از جمله بیماری های شایع در میان جوامع زخم معده است. درمانهای رایج این بیماری شامل داروهای کاهنده ترشح اسید هستند که ضمن اثربخشی دارای عوارض جانبی هستند. استفاده از گیاهان دارویی به عنوان کمک درمان این بیماری مورد توجه است. گیاه دارویی آویشن دنایی Thymusdaenensis به دلیل داشتن ترکیبات متعدد من جمله فلاونوییدها میتواند در پیشگیری از زخم معده موثر باشد. روش تحقیق: برای انجام این مطالعه از 35 موش صحرایی (200-250 گرم)در 7 گروه آزمایشی استفاده شد. گروههای آزمایشی شامل گروه سالین، گروههای دریافت کننده عصارهی آویشن دنایی با دوز 500 و 1000 و 1500 میلی‌گرم/کیلوگرم ، گروه امپرازول با دوز 20 میلی‌گرم/کیلوگرم، گروه کربوکسی متیل سلولز (حلال داروی امپرازول) به صورت خوراکی وگروه شاهد منفی دریافت کننده سالین خوراکی ، بودند. یک ساعت پس از درمان، با خوراندن 5/0 میلی لیتراتانول خالص به هر حیوان به جز گروه آخر زخم معده القا شد. پس از 4 ساعت، حیوانات بیهوش شدند، از قلب خونگیری صورت گرفت و بافت معده جدا گردید و جهت مطالعات هیستوپاتولو‍‍ژیک و بیوشیمیایی مورد استفاده قرارگرفت. میزان سوپراکسیددیسموتاز (SOD) سرم و میزان مالون دی آلدئید (MDA) بافت معده تعیین شد. نتایج و بحث: نتایج در گروههای مورد آزمایش نشان دهنده کاهش آلسر ایندکس وافزایش درصد مهار زخم و اثر محافظتی عصاره گیاه به صورت وابسته به دوز(در دوز بالاتر هم ارز اومپرازول) است. افزایش معنا دار میزان SOD و کاهش معنادار MDA نیز در اثر تجویز عصاره گیاه و اومپرازول بیانگر اثر مفید عصاره بر پایه آثار آنتی اکسیدان آن(کاهش استرس اکسیداتیو) میباشد. توصیه کاربردی/ صنعتی: با توجه به نتایج حاصل می توان از آثار آنتی اکسیدان گیاه آویشن دنایی در درمان بیماریهایی که در اثر عوامل اکسیداتیو ایجاد می گردند در کنار داروهای دیگر بهره برد.
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