Regional variations of antioxidant capacity and phenolic properties in the Iranian jujube collection

Document Type: Original article

Authors

Department of Horticultural Sciences, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

Abstract

Background & Aim: Fruits from the jujube plant (Ziziphus jujuba) possess health-promoting effects and medicinal properties. Jujube has a long history of usage as a remedy in Iranian traditional medicine and is recommended for the treatment of some diseases. This study investigated the variations in antioxidant capacities (AOX), total phenol (TPH), total flavonoid (TF) and total anthocyanin (TA) contents of fruit extracts among 29 jujube accessions originating from 7 provinces of Iran.
Experimental: The fruits from 29 accessions were extracted with methanol (85%). DPPH assay were performed for determination of antioxidant capacity of each extract. The total phenols were measured by Folin-Ciocalteu method and total flavonoids evaluated using the aluminum chloride colorimetric procedure while total anthocyanins were determined by pH-differential method.

Results & Discussion: The results from the analysis of variance (ANOVA) revealed that the examined accessions of Z. jujuba were highly variable in all evaluated phytochemical traits (P <0.01). Principal component analysis (PCA) based on four phytochemical characters confirmed this broad variation and also identified three distinct group among the accessions. The accessions Hamidabad (Mazandaran), Bayaziyeh (Isfahan) and kuhpayeh2 (Isfahan) can considered by their high values for the TPH, TF, and TA values, respectively. On the other hand, accessions Ardestan (Isfahan), Ghaziolya (Ghom) and Maroon (Ghom) showed high AOX. No correlation was found between total phenol content and antioxidant activity.
Industrial and practical recommendations: These findings suggest the importance of genotype and origin for determining antioxidant potential and phenolic contents of jujube plants. The knowledge of the diversities, found in this study, will allow a better plant exploitation related to pharmaceutical uses and also a better conduction of breeding programs for Ziziphus jujubaecotypes.

Keywords


Article Title [Persian]

بررسی تنوع ناحیه ای در کلکسیون عناب ایرانی بر اساس ظرفیت پاداکسایشی و ویژگیهای فنلیک

Authors [Persian]

  • علی عزیزی
  • مریم پیربداقی
گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران
Abstract [Persian]

مقدمه و هدف: میوه های گیاه عناب(Ziziphus jujuba) دارای ویژگیهای سلامتی بخش و خواص دارویی است. استفاده از عناب  به عنوان یک گیاه دارویی برای درمان برخی بیماریها در طب سنتی ایرانی تاریخ دیرینه ای دارد. در این پژوهش تنوع میان 29 نمونه ثبت شده از هفت استان موجود در کلکسیون عناب ایران بر اساس ویژگیهایی شامل ظرفیت پاداکسایشی، محتوای فنل کل، فلاونوئید و آنتوسیانین کل مورد مطالعه قرار گرفت.
روش تحقیق: عصاره گیری از میوه های عناب با استفاده از متانل (85%) انجام شد. آنالیز DPPH جهت اندازه گیری ظرفیت پاداکسایشی مورد استفاده قرار گرفت. جهت سنجش میزان فنل کل، روش مبتنی بر  فولین سیکالتیو به کار رفت و مقدار فلاونوئید کل بر اساس روش رنگ سنجی کلرید آلومنیوم تعیین شد. برای اندازه گیری محتوای آنتوسیانین کل از روش ضریب تغییر اسیدیته استفاده شد.
نتایج و بحث: نتایج آنالیز واریانس نشان داد که نمونه های گیاهی از عناب های مورد آزمایش، یک تفاوت و گوناگونی بالایی(با سطح احتمال ا%) در تمامی صفات فیتوشیمیایی اندازه گیری شده دارند. آنالیز تجزیه به مولفه های اصلی(PCA) بر اساس چهار ویژگی مذکور این تنوع گسترده را تایید کرد و همچنین نمونه های گیاهی را به سه گروه مجزا تفکیک نمود. نمونه گیاهی حمیدآباد ساری (مازندران)، بیاضیه (اصفهان) و کوهپایه 2 (اصفهان) به ترتیب در ویژگیهای محتوای فنل کل، فلاونوئید و آنتوسیانین کل نسبت به بقیه گیاهان برتری داشتند. از طرف دیگر نمونه اردستان (اصفهان)، قاضی اولیا (قم) و مارون (قم) ظرفیت پاداکسایشی بالا داشتند. رابطه همبستگی معنی داری بین محتوای فنل کل و ظرفیت پاداکسایشی یافت نشد.
توصیه کاربردی- صنعتی: یافته های این پژوهش نشان داد که ژنوتیپ و مکان رویش عناب تاثیر زیادی بر پتانسیل پاداکسایشی و ویژگیهای فنلیک آن دارد. آگاهی از تنوع یافت شده در این پژوهش، می تواند در بهره برداری بهتر این گیاه در داروسازی و هدایت بهتر برنامه های بهنژادی عناب کمک نماید.

Keywords [Persian]

  • کلکسیون عناب ایران
  • فنل کل
  • فلاونوئید
  • آنتوسیانین
  • ظرفیت پاداکسایشی
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