Screening of the profiles of the essential oils from the aerial parts of Nepeta racemosa using classical and microwave-based methods: Comparison with the volatiles using headspace solid-phase micro-extraction

Document Type: Original article

Author

Department of Chemistry, Shahrood Branch, Islamic Azad University, Shahrood, Iran

Abstract

Background & Aim:Nepeta racemosa is an herbal and medicinal plant and this report aims to   identify chemical compositions of the essential oils and volatiles of its   aerial parts through classical and advanced methods.  Experimental: Chemical profiles of the essential oils and volatile compounds from the aerial parts of Nepeta racemosa obtained through hydrodistillation (HD), solvent-free microwave extraction (SFME), microwave assisted hydrodistillation (MAHD) and headspace solid-phase microextraction (HS-SPME) methods were subsequently investigated by means of GC and GC-MS instruments.  Results: Totally, 25, 26, 24, and 24 components were identified in the chemical profiles, representing 98.1%, 96.6%, 97.7% and 96.4% of the total compositions when using the HD, HS-SPME, SFME and MAHD methods, respectively. In all samples, oxygenated monoterpenes were the major fractions of the chemical profiles with the exception of chemical composition of the essential oil of N. racemosa obtained by using the MAHD approach in which monoterpene hydrocarbons were dominant constituting compounds. In final, 4aα,7α,7aα-nepetalactone was the most abundant compound in the chemical profiles of HD, HS-SPME and SFME approaches while 4aα,7α,7aβ-nepetalactone oxygenated monoterpene was the most frequent compound in the MAHD profile. Recommended applications/industries: Using the advanced separation methods for isolation of the essential oils are economic, time saving and eminently suited compared with the classical and traditional separation methods. Moreover, the significance of this study relates to the fact that the essential oils, which are rich in monoterpene hydrocarbons and oxygenated monoterpenes could be regarded as powerful food preservatives and novel antioxidants.

Keywords


Article Title [Persian]

بررسی پروفایل روغن های اسانسی حاصل از اندام های هوائی گیاه پونه سای البرزی با استفاده از روش های کلاسیک و روش های مبتنی بر مایکروویو: مقایسه با اجزاء فرار با استفاده از میکرواستخراج فاز جامد فضای فوقانی

Author [Persian]

  • مجید محمدحسینی
گروه شیمی، دانشگاه آزاد واحد شهرکرد، شهرکرد، ایران
Abstract [Persian]

زمینه و هدف: گیاه پونه سای البرزی یک گیاه داروئی بوده و این گزارش به شناسائی ساختارهای شیمیائی روغن های اسانسی و اجزاء فرار حاصل از اندام های هوائی این گیاه با استفاده از روش های سنتی و پیشرفته اختصاص دارد. روش تحقیق: پروفایل های شیمیائی روغن های اسانسی و اجزاء فرار حاصل از اندام های هوائی گیاه پونه سای البرزی با استفاده از روش تقطیر با آب، استخراج مایکروویو بدون حلال، تقطیر با آب به کمک مایکروویو و میکرواستخراج فاز جامد فضای فوقانی، در ادامه با دستگاه های کروماتوگراف گازی و کروماتوگراف گازی- طیف سنج جرمی مورد بررسی قرار گرفتند. نتایج و بحث: مجموعاً، 25، 26، 24 و 24 جزء در پروفایلهای شیمیائی شناسائی شدند که به ترتیب 98/1،96/6، 97/7 و 96/4 درصد کل ساختار را در بر می گیرند. در تمامی نمونه ها (به استثناء پروفایل حاصل از روش تقطیر با آب به کمک مایکروویو) بیشترین فراوانی متعلق به مونوترپن های اکسیژن دار بود در حالیکه در ساختار روغن استحصال شده به روش تقطیر با آب به کمک مایکروویو مونوترپن های هیدروکربنه بیشترین درصد را در اختیار داشتند. ترکیب ۴-آ-آلفا، ۷-آلفا، ۷-آ-آلفا، نپتولاکتون فراوان ترین ترکیب در روش های تقطیر با آب، استخراج مایکروویو بدون حلال و میکرواستخراج فاز جامد فضای فوقانی و ترکیب ۴-آ-آلفا، ۷-آلفا، ۷-آ-بتا نپتولاکتون، به عنوان مونوترپن اکسیژن دار وافر در روش تقطیر با آب به کمک مایکروویو، شناسائی شد. توصیه های صنعتی و عملی: استفاده از روش های جداسازی پیشرفته برای استحصال روغن های اسانسی سودمند، مقرون بصرفه زمانی بوده و در مقایسه با روش های سنتی و کلاسیک بمراتب مناسبتر هستند. بعلاوه، اهمیت این مطالعه، ریشه در این حقیقت دارد که روغن های اسانسی سرشار از مونوترپن های اکسیژن دار و مونوترپن های هیدروکربنه می توانند محافظت کننده های قوی فرآورده های غذائی و آنتی اکسیدان هائی بی نظیر باشند.

Keywords [Persian]

  • پونه سای البرزی
  • تقطیر با آب
  • استخراج مایکروویو بدون حلال
  • تقطیر با آب به کمک مایکروویو
  • میکرواستخراج فاز جامد فضای فوقانی

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