Cytotoxic Effect of Hydroalcoholic Extract from Satureja bachtiarica Bunge on Hela Cancer Cells Line

Document Type: Original article

Authors

1 Department of Biology, Faculty of basic Science, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

2 Department of Medicinal Plants, Shahrekord Branch, Islamic Azad University , Shahrekord, Iran

Abstract

Background & Aim:Cervical cancer is the most common   female genital cancer and the second most common cancer in women in the   world. Since the herbal ingredients have been used for centuries to treat   cancer, purpose of the present study was to determine the cytotoxic   effect of hydroalcoholic extract from Satureja bachtiarica Bungeon   Hela cancer cells line.





Experimental: Hela cells line and natural fibroblast cells line were cultured in RPMI 1640 and DMEM Containing fetal bovine serum and antibiotics, respectively. The cells were exposed to different doses of hydroalcoholic extract of Satureja bachtiarica Bunge (0.156, 0.312, 0.625, 1.25, and 2.5 mg/ml) and were incubated for 24, 48 and 72 hr, respectively. After the incubation period, the modified MTT colorimetric test was used in order to determine cell toxicity of the extract.
 Results: The results of MTT test Showed that hydroalcoholic extract of Satureja bachtiarica Bunge has dose-dependent anti-cancer effect on Hela cancer cells, so that by increasing the concentration of extract and incubation in 72h, the most percentage of cell death was observed (p < 0.05). The extract of plant didn’t show any significant cytotoxicity on natural fibroblast cell line. Therefore, it seems that its compounds will be applied in treating cancer through more researches.
 Recommended applications/industries: Considering the low side effects of herbal medicines, especially antioxidants including Satureja bachtiarica Bunge can be used to treat cancer with drug therapy.

Keywords


Article Title [Persian]

اثر سمیت سلولی عصاره هیدروالکلی مرزه بختیاری بر روی رده سلول های سرطانی Hela

Authors [Persian]

  • آناهیتا اروند شوشتر 1
  • حسین سازگار 1
  • عبداله قاسمی پیربلوطی 2
1 گروه بیولوژی، دانشکده علوم پایه، دانشگاه آزاد اسلامی واحد شهرکرد، شهرکرد، ایران
2 گروه گیاهان داروئی، دانشگاه آزاد اسلامی واحد شهرکرد، شهرکرد، ایران
Abstract [Persian]

مقدمه و هدف: سرطان دهانه رحم شایع­ترین سرطان دستگاه تناسلی زنان و دومین سرطان شایع زنان در دنیاست. با توجه به این­که ترکیبات گیاهی قرن­هاست که برای درمان سرطان استفاده می­شود، هدف از این مطالعه بررسی اثر سمیت سلولی عصاره هیدروالکلی مرزه بختیاری بر روی رده سلول­های سرطانی Hela بود.
روش تحقیق:رده سلول­های سرطانی Hela و رده سلول­های طبیعی فیبروبلاست به ترتیب در محیط کشت RPMI 1640 و DMEM حاوی سرم جنین گاو و آنتی­بیوتیک کشت گردیدند. این سلول­ها در مجاورت دوز­های مختلف عصاره هیدروالکلی مرزه بختیاری (156/0، 312/0، 625/0، 25/1 و 5/2 میلی­گرم بر میلی­لیتر) قرار گرفتند و به مدت 24، 48 و 72 ساعت انکوبه شدند. پس از پایان مدت انکوباسیون، از روش تغییر یافته آزمون رنگ­سنجی MTT جهت تعیین سمیت سلولی عصاره استفاده شد.
نتایج و بحث: نتایج حاصل از تستMTT نشان داد که عصاره هیدروالکلی مرزه بختیاری اثر ضد سرطانی وابسته به دوز و زمان بر سلول­های سرطانی Hela دارد، به طوری که با افزایش غلظت عصاره و انکوباسیون 72 ساعت بیشترین درصد مرگ سلولی مشاهده شد (05/0p<). عصاره گیاه سمیت سلولی قابل توجهی بر روی رده سلول­های طبیعی فیبروبلاست نشان نداد. لذا به نظر می­رسد با تحقیقات بیشتر در آینده می­توان از ترکیبات آن­ در درمان سرطان بهره جست.
توصیه کاربردی/صنعتی: با توجه به اثرات جانبی کم داروهای گیاهی بویژه آنتی­اکسیدان­ها از جمله مرزه بختیاری می­تواند در درمان سرطان در کنار دارو درمانی استفاده شود.

Keywords [Persian]

  • تست MTT
  • مرزه بختیاری
  • Hela
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