Comparative analysis of chemical composition of Mentha longifolia (L.) Huds

Document Type : Original article


1 Department of Agronomy and plant Breeding, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

2 Department of Horticultural, Science and Research Branch, Islamic Azad University, Tehran, Iran

3 Department of Chemistry, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

4 Young Researchers and Elite Club, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran


Background & Aim:Mentha longifolia (L.) Huds is an aromatic perennial herb that belongs to the   family Lamiaceae. The aim of this study was to identify chemical composition of aerial parts   (including leaf, stem and flowers) of M. longifolia collected from   (Kamu mountain) Isfahan province.
Experimental: The essential oils were extracted   using the hydrodistillation method and analysed by GC-MS.
Results: The essential oil yields obtained from the aerial   parts of M. Longifolia were1.34, 0.76 and 0.97 ml/100 g dry   matter in leaf, stem and flowers, respectively. Results indicated significant   differences (p < 0.01) among the aerial parts for the main   constituents in the essential oil. The major constituents of the leaf oil   were 1,8-cineole (37.16%), piperitenone oxide (18.97%), sabinene (13.94%),   α-pinene (8.92%) and pulegone (6.14%). The major constituents of the stem oil   were 1,8-cineole (36.81%), pulegone (18.61%), piperitenone oxide (12.21%),   sabinene (7.05%) and the major constituents of the flower oil were   piperitenone oxide (37.67%), 1,8-cineole (23.02%), sabinene (13.56%) and   α-pinene (10.45%).
 Recommended applications/industries: Differences in the volatile composition of the plant material could be attributed to the growth and cultivation conditions of the plant, to the methods of extraction and to the harvesting time.


Article Title [فارسی]

تغییرات در ترکیبات اسانس پونه کوهی Menthalongifolia(L.) Huds رشد یافته در ایران

Authors [فارسی]

  • احمدرضا گلپرور 1
  • امین هادی پناه 2
  • محمدمهدی قیصری 3
  • سعید صالحی 4
  • رضا خلیلی اذر 1
  • امید قاسمی 1
1 گروه زراعت و اصلاح نباتات، دانشگاه آزاد اسلامی واحد اصفهان (خوراسگان)، اصفهان، ایران
2 گروه باغبانی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات تهران، تهران، ایران
3 گروه شیمی، دانشگاه آزاد اسلامی واحد اصفهان (خوراسگان)، اصفهان، ایران
4 باشگاه پژوهشگران جوان و نخبگان، دانشگاه ازاد واحد کرمانشاه، کرمانشاه، ایران
Abstract [فارسی]

مقدمه و هدف :پونه کوهی با نام علمی (MenthaLongifoliaL.) متعلق به خانواده نعناع (Lamiaceae)می­باشد.هدف از این مطالعه شناسایی عملکرد اسانس و ترکیبات شیمیایی موجود در قسمتهای هوایی گیاه بخصوص برگها، ساقه و گلهای گیاهپونه کوهیدر شرایط آب و هوایی ایران می­باشد.
تحقیق:استخراج اسانس به روش تقطیر با آب و به کمک دستگاه اسانس گیری طرح کلونجر صورت گرفت و میزان اسانس به وسیله دستگاه کروماتوگرافی گازی متصل به طیف سنج جرمی (GC/MS) مورد تجزیه و شناسایی قرار گرفت. تفاوت معنی­داری در عملکرد اسانس از قسمتهای هوایی (برگ، ساقه و گل)  مورد بررسی قرار گرفت.
نتایج و بحث:نتایج بدست آمده از این تحقیق نشان داد که 34/1، 76/0 و 97/0 درصد در اسانس برگ، ساقه و گلهای پونه کوهی به ترتیب شناسایی شد. تفاوت معنی داری در سطح یک درصد در بین ترکیبات شیمیایی بدست آمد. ترکیبات اصلیاسانس برگهای گیاه شامل1,8-cineole(16/37 %)،piperitenone oxide(97/18 %)،sabinene(94/13 %)، α-pinene(92/8 %) و pulegone(14/6 %) بدست آمد.ترکیبات اصلیاسانس ساقه گیاه شامل1,8-cineole(81/36 %)،pulegone(61/18 %)، piperitenone oxide(21/12 %) و sabinene(05/7 %) حاصل شد. ترکیبات اصلیاسانس گلهای گیاه شاملpiperitenone oxide(67/37 %)،1,8-cineole(02/23 %)،sabinene(56/13 %) و α-pinene(45/10 %)بدست آمد.
توصیه کاربردی/صنعتی:خصوصیات مورفولوژیکی می­تواند تحت شرایط فاکتورهای مختلف یعنی محیطی و ژنتیکی قرار گیرد.

Keywords [فارسی]

  • ترکیب شیمیایی
  • اسانس
  • Mentha longifolia (L.) Huds
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