An in vitro study on anti-sickling activity of Terminalia arjuna and Terminalia bellirica

Document Type: Original article

Authors

Human Genetic Lab, Centre for Scientific Research and Development, Peoples University, Bhopal-462037, Madhya Pradesh, India

Abstract

Background & Aim:Combretaceae family is   known for efficacy in the management of sickle cell disorder (SCD). Based on   the earlier reports of anti-sickling property of Terminalia genus the study investigates in vitro antisickling properties of Terminalia arjuna and Terminalia   bellirica.
Experimental: Anti-sickling   assay of methanolic and aqueous extract of T. arjuna and T. bellirica   on sickled RBCs were evaluated at different concentrations. The osmotic   fragility test was done to explore the effect of extracts on haemoglobin S   solubility and sickle cell membrane stability. Additionally antioxidant   activity and phytochemical analysis of extracts was evaluated.





Results: Treatment with the plant extracts at different concentrations showed decrease in percentage of sickled cells. Extract of T. arjuna had a significantly higher (p < 0.05) antisickling activity at the tested concentrations of 10 mg/ml. The methanolic extract at 10 mg/ml showed the best cell membrane stability as compared to T. bellirica extract. It was observed that, T. bellirica extract was deficient of alkaloid and tannins. Moreover, all extracts/fractions showed antioxidant activity, although lesser as compared to the standard.
Recommended applications/industries: It was observed that the leaf extracts of T. arjuna has a significant antisickling effect, hence it can be potentially used in clinical management of SCD. The bioactive compounds can be used to supplement as well as management drug for sickle cell disease.

Keywords

Article Title [Persian]

مطالعه آزمایشگاهی بر روی فعالیت ضد داسی شدن سلولی در Terminalia arjuna و Terminalia bellirica

Authors [Persian]

  • آناند دیلید فیرودییا
  • آبین مانی
  • ویجای تاوانی
Abstract [Persian]

Background & Aim:Combretaceae family is   known for efficacy in the management of sickle cell disorder (SCD). Based on   the earlier reports of anti-sickling property of Terminalia genus the study investigates in vitro antisickling properties of Terminalia arjuna and Terminalia   bellirica.
Experimental: Anti-sickling   assay of methanolic and aqueous extract of T. arjuna and T. bellirica   on sickled RBCs were evaluated at different concentrations. The osmotic   fragility test was done to explore the effect of extracts on haemoglobin S   solubility and sickle cell membrane stability. Additionally antioxidant   activity and phytochemical analysis of extracts was evaluated. 

Results: Treatment with the plant extracts at different concentrations showed decrease in percentage of sickled cells. Extract of T. arjuna had a significantly higher (p < 0.05) antisickling activity at the tested concentrations of 10 mg/ml. The methanolic extract at 10 mg/ml showed the best cell membrane stability as compared to T. bellirica extract. It was observed that, T. bellirica extract was deficient of alkaloid and tannins. Moreover, all extracts/fractions showed antioxidant activity, although lesser as compared to the standard.
Recommended applications/industries: It was observed that the leaf extracts of T. arjuna has a significant antisickling effect, hence it can be potentially used in clinical management of SCD. The bioactive compounds can be used to supplement as well as management drug for sickle cell disease.

Keywords [Persian]

  • Sickle cell disease
  • methanolic extract
  • Phytochemical
  • Osmotic fragility
  • Antioxidant

References

Moody, J.O., Ojo, O.O., Omotade, A.A., Adeyemo, A.A., Olumese, P.E. and Ogundipe, O.O. 2003. Anti-sickling potential of Nigerian Herbal Formula (Ajawaron HF) and the major plant component (Cissus populnea L. CPK). Phytotherapy Research, 17(10): 1173-1176.

Mpiana, P.T., Tshibangu, D.S.T., Shetonde, O.M. and Ngbolua, K.N. 2007. In vitro antidrepanocytary activity (anti-sickle cell anaemia) of some Congolese plants. Phytomedicine, 14(2-3): 192-195.

Mandot, S., Khurana, V.L. and Sonesh, J.K.  2009. Sickle Cell Anemia in Garasia Tribals of Rajasthan. Indian Pediatrics, 46(3): 239-340.

Mpiana, P.T., Lombe, B.K., Ombeni, A.M., Ngbolua, K.T.N., Tshibangu, D.S.T., Wimba LK, et al. 2013. In vitro sickling inhibitory effects and antisickle erythrocytes hemolysis of Dicliptera colorata CB Clarke, Euphorbia hirta L and Sorghum bicolor (L.) Moench. Open Journal of Blood Diseases, 3: 43-48.

Clarke, D.P. and Pazdernik, N.J. 2013. Molecular biology. Waltham, USA: Elsevier Inc: p. 417-57.

Mpiana, P.T., Ngbolua, K.N., Mudogo, V., Tshibangu, D.S.T., Atibu, E.K., Tshilanda, D.D., et al. 2011. Anti-sickle erythrocytes haemolysis properties and inhibitory effect of anthocyanins extracts of Trema orientalis (Ulmaceae) on the aggregation of human deoxyhemoglobin S in vitro. Journal of Medical Sciences, 11(3): 129-37.

Makani, J., Ofori-Acquah, S.F., Nnodu, O., Wonkam, A. and Ohene-Frempong K. 2013. Sickle cell disease: new opprtinuities and challenges in Africa. The Scientific World Journal. 1-16.

Mpiana, P.T.,  Ngbolua, K.N.N.,  Bokota, M.T.,  Kasonga, T.K., Atibu, E.K.,  Tshibangu., et al. 2010. In vitro effects of anthocyanin extracts from Justicia secunda Vahl on the solubility of haemoglobin S and membrane stability of sickle erythrocytes. Blood Transfusion. 8(4): 248–254.

Pietrovski, E.F., Rosa, K.A., Facundo, V.A., Rios, K., Marques, M.C.A. and Santos, A.R.S. 2006. Antinociceptive properties of the ethanolic extract and of the triterpene 3β,6β,16β-trihidroxilup-20(29)-ene obtained from flowers of Combretum leprosum in mice. Pharmacology Biochemistry and Behavior. 83(1):90–9.

Cock, I.E. 2015. The medicinal properties and phytochemistry of plants of the genus Terminalia (Combretaceae). Inflammopharmacology. 23(5):203-29.

Gangarde, S.K., Tripathi, N.K., Harinkhede, D.K. and Mishra, P.K. 2003. Ethno-medicinal diversity used by tribal’s of central India, Sam Com Digital Graphics, Indore.

Adesanya, S.A. and Sofowora, A. 1983. Biological standardization of Zanthoxylum roots for antisickling activity. Plant Medica. 48(5): 27-33.

Harbone, J,B. 1998. Phytochemical methods: A guide to modern techniques of plant analysis. 3rd Edition. Chapman and Hill, London. p. 279.

Egunyomi, A., Moody, J.O. and Eletu, O,M. 2009. Antisickling activities of two ethnomedicinal plant recipes used for the management of sickle cell anaemia in Ibadan, Nigeria. African Journal of Biotechnology. 8(1): 20-25.

Jaja, S.I., Kehinde, M.O., Gbenebitse, S., Mojiminyi, F.B.O. and Ogungbemi, A.I. 2000. Effect of vitamin C on arterial blood pressure, irreversible sickled cells and osmotic fragility in sickle cell anemia subjects. Nigerian journal of physiological sciences. 16:14–18.

Sofowora, E.A., Isaac-Sodeye, W.A. and Ogunkoya, L.O. 1979. Isolation and characterization of an antisickling agent from the root of Fagara xanthoxyloides. African medicinal plants. Proceedings of a Conference. Eds. Sofowora EA. pp. 89-97.

Egunyomi, A., Moody, J.O. and Eletu, O.M. 2009. Antisickling activities of two ethnomedicinal plant recipes used for the management of sickle cell anaemia in Ibadan, Nigeria. African Journal of Biotechnology. 8: 20-25.

Oduola, T., Adeniyi, F.A.A., Ogunyemi, E.O., Bello, I.S. and Idowu, T.O. 2006. Antisickling agent in an extract of unripe pawpaw (Carica papaya): Is it real? African Journal of Biotechnology. 5(20): 1947-9.

Choi, C.W., Kim, S.C., Hwang, S.S., Choi, B.K., Ahn, H.J., Lee, M.Y., Park, S.H. and Kim, S.K. 2002. Antioxidant activity and free radical scavenging capacity between Korean medicinal plants and flavonoids by assay-guided comparison. Plant Science. 163(6): 1161–1168.

Robert, M.F. and Wink, M. 1998. Alkaloids: biochemistry, ecology, and medicinal applications. New York: Plenum Press.

Middleton, E. Jr. and Kandaswami, C. 1992. Effects of flavonoids on immune and inflammatory cell functions. Biochem Pharmacol. 43(6):1167-1179.

Kenner, D.L., Yves, R.M.D. 1996. Botanical Medicine. A European Professional Perspective. p. 487.

Gillete, P.N., Misra, R., Wambebe, C., Asakura, T. and Pandey, R.C. 2004. Development of Niprisan, an antisickling phytopharmaceutical from traditional medicine: Lessons learned and challenges for the future. Fourth International Healthcare and Herbal Expo and Seminar, Source Book April 2-4, New Delhi, India. pp. 141-149.

Rai, D.K., Rai, P.K., Rizui, S., Watal, G. and Shama, B. 2009. Carbofuran Induced Toxicity in Rats: Protective Role of Vitamin C. Experimental and Toxicologic Pathology. 61(6): 531-535.

Miliauskas, G., Venskutonis, P.R. and van Beek, T.A. 2004. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chemistry. 85(2): 231-237.

 

Journal of Herbal Drugs (An International Journal on Medicinal Herbs)

Volume 8, Issue 1
Spring 2017
Pages 1-7

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