An Investigation of Compositions and Effects of Local Herbal Glycyrrhiza glabra and Mentha pulegium extracts on Helicobacterpylori and Cell Line of stomach Cancer (AGS) by MTT assays

Document Type: Original article

Authors

1 Department of Biology, Ardabil Branch, Islamic Azad University, Ardabil, Iran

2 Department of Biology, Ardabil Branch, Islamic Azad University, Ardabil, Iran;

Abstract

Background & Aim:According to globally development of stomach cancer especially in Ardabil, Iran, as the second major cause of mortality throughout the world, increased drug-resistant bacteria including Helicobacter pylori as the most important risk factors for stomach cancer, and side effects of antibiotics and chemical drugs normally used to treat.
Experimental: The current research was conducted to investigate the anticancer and antimicrobial effects of native herbs of liquorice (Glycyrrhiza glabra) and pennyroyal (Mentha pulegium) extractions for finding a solution with the lowest complications in control or treatment of stomach cancer.The extractions were firstly obtained using Soxhlet and methanol solvent and then their compounds were determined by GC/MS. Antimicrobial activity, MIC and MBC of the extractions were assessed respectively using agar diffusion and broth dilution test and the anticancer effect on stomach cancer (cell line AGS) was assessed by MTT assay. H. pylori ATCC 26695 was respectively revived and purified on Brucella broth containing 7% citrated horse serum and Columbia agar.
Results: The analysis showed thatliquorice extract contains 15 compositions, mainly consists of 26.48% Nonane, 23.38% Ethylcyclohexan, 8.29% 3-Bromodecane, 10.31% trans-2-Heptenal, 8.93% 9-Octadecenamide and 4.68% β-pregna and pennyroyal extract contains 17 compositions, mainly including 3.36% Camphor, 22.79% Pulegone, 4.92% Paramenth-3-n8-l, 8.06% Menthoforan, 7.54% Cis-Isopulegon and 24.58% α-Selinene. The bacteria were resistant or semi-sensitive to common antibiotics, whereas had considerable sensitivity to herbal extracts and liquorice showed almost three times more antibacterial effect. Pennyroyal extract had no cytotoxic effects, but the anticancer effect was observed in liquorice extract with optimal concentration of 25 μg/ml after 48 hours.
Recommended applications/industries: In conclusion, liquorice extract due to the significant health benefits of anticancer and antibacterial activity can be selective and highly effective herbal medicine as an alternative to antibiotics and other chemical drugs.

Keywords


Article Title [Persian]

بررسی ترکیبات شیمیایی و تاثیرات عصاره گیاهان دارویی خارمریم (Silybum marianum) و رازیانه (Foeniculum vulgare) بر برخی عوامل عفونت‌های بیمارستانی و رده سلول سرطانی کبد (HepG2) به روش MTT

Authors [Persian]

  • فرشته سلمانی 1
  • هاشم یعقوبی 2
1 گروه بیولوژی، واحد اردبیل، دانشگاه ازاد اسلامی، اردبیل ، ایران
2 گروه بیولوژی، واحد اردبیل، دانشگاه ازاد اسلامی، اردبیل ، ایران
Abstract [Persian]

مقدمه و هدف: افزایش مقاومت دارویی و توجه بیشتر به خطرات عوارض جانبی داروهای صناعی باعث شده است که تحقیقات گسترده ای بر روی منابع گیاهی دارویی انجام شود. هدف از انجام این مطالعه تعیین ترکیبات شیمیایی عصاره گیاهان دارویی خار مریم (Silybum marianum) و رازیانه (Foeniculum vulgare) بومی اردبیل و بررسی اثر ضد سرطان آنها بر روی رده سلولی سرطان کبد HepG2 و اثرات ضد باکتریایی آنها بر روی استافیلوکوکوس اورئوس و سودوموناس آئروژینوزا جهت ارائه یک راه حل برای کنترل یا درمان بود.
روش تحقیق: ترکیبات شیمیایی عصاره های به دست آمده از طریق دستگاه سوکسیله توسط سیستم GC/MS انجام گرفت. اثر ضد سرطانی با روش MTT و فعالیت های ضد میکروبی با استفاده از روش انتشار دیسک مورد سنجش قرار گرفتند و همچنین مقادیر MIC و MBC بر اساس روش رقت در لوله برآورد گردید.
نتایج و بحث: عصاره خارمریم با 29 ترکیب و عمدتا شامل 63/2% Nonane، 68/4% Camphor، 84/2% Borneol، 65/3% Trans-anethole، 78/8% Viridiflorol، 26/34% α–Selinene، 34/2% Longiborn-9-ene، 52/2% amorphane-B، 00/5% Erucylamide، 15/10% o-Terphenyl و 8/46% α-androst-8-ene بود، عصاره رازیانه با 14 ترکیب و عمدتا شامل 53/5 درصد Fenchone، 53/5% α- Thujone، 84/2% Nonane، 10/79% trans-Anethol و 51/2 درصد Hentriacontane بود. اثرات عصاره رازیانه بر روی استافیلوکوکوس و عصاره خارمریم بر روی سودوموناس قابل توجه بود. در طرح ترکیبی، تاثیر خاصی مشاهده نشد. مقادیر MIC و MBC نتایج حاصل از تست حساسیت را تایید کردند. اثرات سیتوتوکسیک عصاره‌ها بر رده سلول سرطانی کبد در غلظت‌ 25 µg/ml بعد از 48 ساعت اپتیمم بود.
توصیه کاربردی/صنعتی: با توجه به نتایج، این ترکیبات می‌توانند جایگزین مناسبی برای مواد نگهدارنده شیمیایی در صنایع غذایی و صنایع دارویی باشند.

Keywords [Persian]

  • خار مریم
  • رازیانه
  • استافیلوکوکوس اورئوس
  • سودوموناس آئروجینوزا
  • HepG2
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