Chemical composition and antimicrobial activity of essential oil of Salvia officinalis L. and Salvia virgata Jacq

Document Type: Original article

Authors

1 Department of Agronomy and plant Breeding, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

2 Department of Horticultural Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran

3 Toxicology Research Center, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

4 Department of Horticulture, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

5 Department of Horticultural Sciences, Yasouj Branch, Islamic Azad University, Yasouj, Iran

6 Department of Horticultural Sciences, Hormozgan University, Bandar Abbas, Iran

Abstract

Background & Aim:The genus Salvia (commonly known as sage) is a broad genus belonging to the family Lamiaceae. This study is aimed at assessing the essential oil composition and antimicrobial activities of two Salvia species.
Experimental: The chemical composition of the essential oils of Salvia officinalis L. and Salvia virgata Jacq, cultivated in Estahban (Fars, South Iran), was studied by means of GC-MSanalyses. Antimicrobial activity was tested against a panel of microorganisms including one Gram-positive (Staphylococcus epidermidis PTCC NO. 1435) and one Gram-negative (Escherichia coli) and three fungal strain (Alternaria alternata PTCC NO. 5224, Penicillium funiculosum PTCC NO. 5301 and P. funiculosum PTCC NO. 5169) using the disk diffusion and agar-well diffusion methods and the Minimum Inhibitory Concentration (MIC) technique.
Results: In all, 57 compounds were identified, 42 for S. officinalis, accounting for 98.94% ofthe total oil, 29 for S. virgata (98.81%). The major components of S. officinalis essential oil were α-thujone (37.18%), 1,8-cineole (12.71%), β-thujone (9.10%) and the major components of S. virgata essential oil were caryophyllene oxide (30.23%), β-caryophyllene (22.63%), sabinene (11.82%). The antimicrobial activity of the total essential oil evaluated by the agar-well diffusion method, the results showed that the highest active against S. epidermidis and also the least active against E. coli. Inhibition of growth was tested by the disk diffusion method, the results showed that essential oil of S. officinalis and S. virgata were highest active against E. coli and P. funiculosum (PTCC NO. 5301), respectively. Also the least active against A. alternata.
Recommended applications/industries: The results showed thatS. officinalis oil had higher antimicrobial activity compare to S. virgata

Keywords


Article Title [Persian]

ترکیبات شیمیایی و فعالیت ضد میکروبی اسانس (Salvia officinalis L) و (Salvia virgata Jacq)

Authors [Persian]

  • احمدرضا گلپرور 1
  • امین هادی پناه 2
  • محمدمهدی قیصری 3
  • داوود نادری 4
  • شیما رحمانیان 5
  • مجتبی خرمی 6
1 گروه زراعت و اصلاح نباتات، واحد خوراسگان، دانشگاه آزاد اسلامی، اصفهان، ایران
2 گروه علوم باغی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
3 مرکز تحقیقات سم شناسی، واحد خوراسگان، دانشگاه آزاد اسلامی، اصفهان، ایران
4 گروه باغبانی، واد خوراسگان، دانشگاه آزاد اسلامی، اصفهان، ایران
5 گروه علوم باغی، واحد یاسوج، دانشگاه آزاد اسلامی، یاسوج، ایران
6 گروه علوم باغی، دانشگاه هرمزگان، بندرعباس، ایران
Abstract [Persian]

مقدمه و هدف : (Salvia officinalis L.) و (Salvia virgata Jacq) گیاهان چند ساله و معطر از خانواده نعناع (Lamiaceae) می­باشد. هدف از این تحقیق شناسایی ترکیبات شیمیاییو فعالیت ضد میکروبی دو گونه Salvia بود.
روش تحقیق: قسمتهای هوایی S. officinalis و S. virgata  به وسیله دستگاه کروماتوگرافی گازی متصل به طیف سنج جرمی (GC/MS) مورد تجزیه و شناسایی قرار گرفت. خاصیت ضد باکتریایی با استفاده از سویه باکتری­های استاندارد شامل Staphylococcus epidermidisوEscherichia coliو قارچ های Alternaria alternataوPenicillium funiculosumدردو روش دیسک دیفیوژن و چاهک گذاریمورد بررسی قرار گرفت.
نتایج و بحث: نتایج بدست آمده از این تحقیق نشان داد که 42 ترکیب در اسانس S. officinalis و 29 ترکیب در اسانس S. virgata  به  ترتیب شناسایی شد. ترکیبات اصلی اسانس S. officinalis شامل α-thujone  (18/37%)، 1,8-cineole  (71/12%)، β-thujone  (10/9%)، camphene (54/5%) و viridiflorol  (33/5%) شناسایی شد. و ترکیبات اصلی اسانس S. virgata  شامل caryophyllene oxide (23/30%)، β-caryophyllene (63/22%)، sabinene (82/11%)، 1-octan-3-ol (64/6%)، thujene (28/6%) و terpinene-4-ol (25/5%) شناسایی شد. اسانس  S. officinalisو S. virgata   در روش چاهک گذاری بیشترین ویژگی ضد میکروبی را برای S. epidermidisنشان داده و کمترین اثر را بر E. coli داشت. در روش دیسک دیفیوژن  بیشترین ویژگی ضد میکروبی اسانس  S. officinalisو S. virgata   بر ضد  E. coliوP. funiculosum (PTCC NO. 5301) بوده و کمترین اثر را بر A. alternate  نشان داد.
توصیه کاربردی/صنعتی: در مجموع اسانس S. officinalisخاصیت ضد میکروبی بیشتری نسبت به گیاهS. virgata   نشان داد.

Keywords [Persian]

  • Salvia officinalis L
  • Salvia virgata Jacq
  • ترکیبات شیمیایی
  • خاصیت ضد میکروبی
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