Study of phytohormones effects on UV-B stress seeds of thyme species

Document Type: Original article

Author

Department of Agronomy and Medicinal Plants, Faculty of Agriculture, Shahrekord Branch, Islamic Azad University, Po.Box:166. Shahrekord, Iran.

Abstract

Background & Aim: Thymus vulgaris L. and Thymus daenensis Celak (Thyme), members of the family Lamiaceae, are widely used in Iranian folk medicine. The aim of this research was to study how salicylic acid (SA), gibberellin (GA), and indole acetic acid (IAA)-seed priming affect UV-B radiation in seeds of Thyme (T. vulgaris, T. daenensis Celak) under both laboratory and outdoor conditions.
Experimental: The effects of phytohormones (200, 400, 600 ppm) and irradiation performed in a 60 Co Gamma cell 220 source at a dose of 3 kGy (10, 20 and 30 min) on seeds of Thyme species were studied. Seeds were tested under in vitro and outdoor conditions in a complete randomized factorial layout with 4 and 3 replications, respectively. The characters measured under in vitro condition were seed germination percentage, mean time of germination, root and stem length. On the field, the characters evaluated were number of leaf, length of plant, root and shoot dry matter and essential oil.
Results: SA treatment was better under in vitro conditions. No significant effects were obtained from GA and IAA. The most destructive effects and the best beneficial phytohormones were UV 30 min and SA (200 and 400 ppm), respectively. The effects of foliar application of hormones were determined on growth and essential oil production in medicinal plants in two consecutive years. Shoot dry matter increased in both years with SA applications but IAA and GA had no beneficial effects on germination characters after UV radiation.
Recommended applications/industries: SA-priming of seeds protects thyme seedlings against UV-B radiation. The results of this study also showed that elevation of free SA levels in plants, either by exogenous feeding or genetically may enhance their tolerance to abiotic stress.

Keywords


Article Title [Persian]

مطالعه اثر فیتوهورمون ها بر بذور گونه های آویشن تحت استرس اشعه فرابنفش.

Author [Persian]

  • مهراب یادگاری
گروه گیاهان داروئی، دانشکده کشاورزی، دانشگاه ازاد واحد شهرکرد، شهرکرد، ایران
Abstract [Persian]

پیش زمینه و اهداف: Thymus vulgaris L. وT.daenensiscelakدو گونه دارویی چندساله متعلق به خانواده نعناعیان می باشند. هدف این تحقیق مطالعه اثرات پرایمینگ بذور دو گونه آویشن  Thymus vulgaris L. وT.daenensiscelakتحت تنش اشعه فرابنفش با سالیسیلیک اسید، جیبرلیک اسید و ایندول استیک اسید در شرایط آزمایشگاهی و مزرعه ای بود.
شرایط آزمایشی: بذور تحت تیمار با هورمون‌های گیاهی با دوزهای 200، 400 و 600 ppmو اشعه فرابنفش با 220 گاماسل و شدت 3 kGy در سه زمان 10، 20 و 30 دقیقه قرار گرفتند. طرح آزمایشی در آزمایشگاه به صورت کاملاً تصادفی به صورت فاکتوریل و در مزرعه به صورت کاملاً تصادفی با سه تکرار انجام شد. صفات مورد برآورد در آزمایشگاه شامل درصد جوانه­زنی، متوسط زمان جوانه­زنی، طول ریشه­چه و ساقه­چه و در شرایط مزرعه تعداد برگ، ارتفاع گیاه، وزن خشک ریشه و اندام هوایی و میزان اسانس بود.
نتایج و بحث:نتایج در تمامی گیاهان نشاندهنده آن بود که در شرایط آزمایشگاه، سالیسیلیک اسید بهتر از سایر تیمارها بود و تفاوت معنی‌داری در مورد اثرات جیبرلیک اسید و ایندول استیک اسید، دیده نشد. بیشترین اثرات مخرب و بهترین اثرات مفیدبه ترتیب بواسطه تابش اشعه فرابنفش به مدت 30 دقیقه و هورمون سالیسیلیک اسید با غلظت 200 و 400 ppm بدست آمد. اثرات کاربرد هورمون‌ها توسط رشد و عملکرد گیاهان در دو سال متمادی تعیین شد. وزن خشک اندام هوایی در هر دو سال بواسطه کاربرد سالیسیلیک اسید افزایش یافت اما ایندول استیک اسید و جیبرلیک اسید تأثیر سودمندی بر بذور تحت تنش اشعه فرابنفش نداشتند.
توصیه کاربردی/ صنعتی: پرایمینگ با سالیسیلیک اسید بر خلاف ایندول استیک اسید و جیبرلیک اسید، منجر به محافظت بذور تحت تنش اشعه فرابنفش می شود. نتایج این تحقیق نشاندهنده آنست که افزایش سالیسیلیک اسید در گیاهان، چه به صورت کاربرد خارجی چه به صورت طبیعی منجر به تحمل به تنش های غیرزنده می شود.

Keywords [Persian]

  • هورمون های گیاهی
  • گونه های آویشن
  • تنش اشعه فرابنفش
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