Chemical Composition and Bioactivity of the Essential Oil of Melissa officinalis L., Cultivated in Southwestern, Iran

Document Type: Original article

Authors

Department of Medicinal Plants, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran

Abstract

Background & Aim: Melissa officinalis L. (lemon balm) a valuable medicinal plant in herbal medicine is native to the eastern Mediterranean region and western Asia. It is a traditional herbal medicine, which enjoys contemporary usage as a mild sedative, spasmolytic and antibacterial agent. The objective of this study was to assess the effects of climate on quality and quantity of the essential oil of M. officinalis at Shahrekord climate, southwestern (Iran) and evaluate the antioxidant activity of the oil.
Experimental: Plants were cultivated during 22-25th March 2016 in the greenhouse and transferred to the main site after three months (May), and finally harvested at the full flowering stage during August.Dried plant material (100 g) was powdered and subjected to hydro–distillation for three hours using a Clevenger–type apparatus. The hydrodistillated essential oil analyzed by GC-FID and GC/MS. The antioxidant activity was determined by using DPPH method.
Results: Results indicated that the major essential oil constituents of M.officinalis L. were β-caryophyllene (23.06%), E-citral (17.61%), Z-citral (13.64%), and caryophyllene oxide (10.83%). The antioxidant activityof the essential oil showed moderate antioxidant activity (IC50=749.60 µg/g), that was lower compared to butylated hydroxytoluene (BHT).
Recommended applications/industries: This potential applicability can be used as antioxidant agents for food and pharmaceutical industries.

Keywords


Article Title [Persian]

ترکیب شیمیایی و فعالیت زیستی اسانس Melissa officinalis کشت شده در جنوب غربی ایران

Authors [Persian]

  • هاله محمدپور
  • عبدالله قاسمی پیربلوطی
  • فاطمه ملک پور
  • بهزاد حامدی
گروه گیاهان دارویی، دانشگاه آزاد اسلامی، واحد شهرکرد، شهرکرد، ایران
Abstract [Persian]

Background & Aim: Melissa officinalis L. (lemon balm) a valuable medicinal plant in herbal medicine is native to the eastern Mediterranean region and western Asia. It is a traditional herbal medicine, which enjoys contemporary usage as a mild sedative, spasmolytic and antibacterial agent. The objective of this study was to assess the effects of climate on quality and quantity of the essential oil of M. officinalis at Shahrekord climate, southwestern (Iran) and evaluate the antioxidant activity of the oil.
Experimental: Plants were cultivated during 22-25th March 2016 in the greenhouse and transferred to the main site after three months (May), and finally harvested at the full flowering stage during August.Dried plant material (100 g) was powdered and subjected to hydro–distillation for three hours using a Clevenger–type apparatus. The hydrodistillated essential oil analyzed by GC-FID and GC/MS. The antioxidant activity was determined by using DPPH method.
Results: Results indicated that the major essential oil constituents of M.officinalis L. were β-caryophyllene (23.06%), E-citral (17.61%), Z-citral (13.64%), and caryophyllene oxide (10.83%). The antioxidant activityof the essential oil showed moderate antioxidant activity (IC50=749.60 µg/g), that was lower compared to butylated hydroxytoluene (BHT).
Recommended applications/industries: This potential applicability can be used as antioxidant agents for food and pharmaceutical industries

Keywords [Persian]

  • Melissa officinalis
  • DPPH
  • ترکیب شیمیایی
  • GC-MS
Adams, R.P. 2001. Identification of essential oils by gas chromatography quadrupole mass spectroscopy. Allured: Carol Stream, IL.

Ali, M.S., Saleem, M., Ali, Z. and Ahmad, V.U. 2000. Chemistry of Zataria multiflora (Lamiaceae). Phytochemistry, 55: 933–936.

Bajalan, I. and Ghasemi Pirbalouti, A. 2014. Variation in antibacterial activity and chemical compositions of essential oil from different populations of myrtle. Industrial Crops and Products, 61: 303–307.

Bajalan, I., Rouzbahani, R., Ghasemi Pirbalouti, A. and Maggi, F. 2017. Chemical composition and antibacterial activity of Iranian Lavandula x hybrida. Chemistry and Biodiversity, 14: e1700064.

Basta, A., Tzakou, O. and Couladis, M. 2005. Composition of the leaves essential oil of Melissa officinalis from Greece. Flavour and fragrance journal, 20: 642–644.

Blumenthal, M., Goldberg, A. and Brinckmann, J. 2000. Herbal Medicine-Expanded Commission E Mongraphs. Newton, MA: Integrative Medicine Communications, 123: 230-232.

Bounihi, A., Hajjaj, G., Alnamer, R., Cherrah, Y. and Zellou, A. 2013. In vivo potential anti-inflammatory activity of Melissa officinalis L. essential oil. Advances in pharmacological sciences, 2013; 1–7.

Encalada, M.A., Hoyos, K.M., Rehecho, S., Berasategi, I., de Ciriano, M.G.Í., Ansorena, D., Astiasarán, I., Navarro-Blasco, Í., Cavero, R.Y. and Calvo, M.I. 2011. Anti-proliferative effect of Melissaofficinalis on human colon cancer cell line. Plant foods for human nutrition, 66: 328–334.

Erdemoglu, N., Turan, N.N., Cakõcõ, I., Sener, B. and Aydõn, A. 2006. Antioxidant activities of some Lamiaceae plant extracts. Phytotherapyresearch, 20: 9–13.

Fecka, I., Raj, D. and Krauze-Baranowska, M. 2007. Quantitative determination of four water-soluble compounds in herbal drugs from Lamiaceae using different chromatographic techniques. Chromatographia, 66:87–93.

Gülçin, I., Elmastaş, M. and AboulEnein, H.Y. 2007. Determination of antioxidant and radical scavenging activity of Basil (Ocimumbasilicum L. Family Lamiaceae) assayed by different methodologies. Phytotherapy research, 21: 354–361.

Jan, S., Khan, M.R., Rashid, U. and Bokhari, J. 2013. Assessment of antioxidant potential, total phenolics and flavonoids of different solvent fractions of Monotheca buxifolia fruit. Osong public health and research perspectives, 4: 246–254.

Hung, D., Ou, B. and Prior, R.L. 2005. The chemistry behind antioxidant capacity assays. Journal of Agricultural and Food Chemistry, 53:1841–56.

Kennedy, D.O., Little, W. and Scholey, A.B. 2004. Attenuation of laboratory-induced stress in humans after acute administration of Melissaofficinalis (Lemon Balm). Psychosomatic medicine, 66: 607–613.

Koksal, E., Bursal, E., Dikici, E., Tozoglu, F. and Gulcin, I. 2011. Antioxidant activity of Melissa officinalis leaves. Journal of Medicinal Plants Research, 5: 217–222.

Koşar, M., Dorman, H. and Hiltunen, R. 2005. Effect of an acid treatment on the phytochemical and antioxidant characteristics of extracts from selected Lamiaceae species. Food Chemistry, 91: 525–533.

Matkowski, A., Tasarz, P. and Szypuła, E. 2008. Antioxidant activity of herb extracts from five medicinal plants from Lamiaceae, subfamily Lamioideae. Journal of Medicinal Plants Research, 2: 321–330.

Mimica-Dukic, N., Bozin, B., Sokovic, M. and Simin, N. 2004. Antimicrobial and antioxidant activities of Melissaofficinalis L.(Lamiaceae) essential oil. Journal of agricultural and food chemistry, 52: 2485–2489.

Özguven, M. and Tansi, S. 1998. Drug yield and essential oil of Thymusvulgaris L. as in influenced by ecological and ontogenetical variation. Turkish Journal of Agriculture and Forestry, 22: 537–542.

Papadopoulos, A., Luo, X., Leonhart, S., Gosselin, A., Pedneault, K., Angers, P., Gaudreau, L. and Dorais, M. 2000. Soilless greenhouse production of medicinal plants in North Eastern Canada, In: World Congress on Soilless Culture: Agriculture in the Coming Millennium, 554:  297–304.

Pereira, R.P., Boligon, A.A., Appel, A.S., Fachinetto, R., Ceron, C.S., Tanus-Santos, J.E., Athayde, M.L. and Rocha, J.B.T. 2014. Chemical composition, antioxidant and anticholinesterase activity of Melissa officinalis. Industrial Crops and Products, 53: 34–45.

Ghasemi Pirbalouti, A., Hashemi, M. and Ghahfarokhi, F.T. 2013. Essential oil and chemical compositions of wild and cultivated Thymusdaenensis Celak and Thymus vulgaris L. Industrial Crops and Products, 48: 43–48.

Roby, M.H.H., Sarhan, M.A., Selim, K.A.H. and Khalel, K.I. 2013. Evaluation of antioxidant activity, total phenols and phenolic compounds in thyme (Thymus vulgaris L.), sage (Salvia officinalis L.), and marjoram (Origanum majorana L.) extracts. Industrial Crops and Products, 43: 827–831.

Sadraei, H., Ghannadi, A. and Malekshahi, K. 2003. Relaxant effect of essential oil of Melissa officinalis and citral on rat ileum contractions. Fitoterapia, 74: 445–452.

Sarikurkcu, C., Arisoy, K., Tepe, B., Cakir, A., Abali, G. and Mete, E. 2009. Studies on the antioxidant activity of essential oil and different solvent extracts of Vitexagnuscastus L. fruits from Turkey. Food and Chemical Toxicology, 47: 2479–2483.

Sousa, A.C., Gattass, C.R., Alviano, D.S., Alviano, C.S., Blank, A.F. and Alves, P.B. 2004. Melissa officinalis L. essential oil: antitumoral and antioxidant activities. Journal of pharmacy and pharmacology, 56: 677–681.

Spiridon, I., Colceru, S., Anghel, N., Teaca, C.A., Bodirlau, R. and Armatu, A. 2011. Antioxidant capacity and total phenolic contents of oregano (Origanumvulgare), lavender (Lavandula angustifolia) and lemon balm (Melissa officinalis) from Romania. Natural product research, 25: 1657–1661.

Tabatabaei, S.J. 2008. Effects of cultivation systems on the growth, and essential oil content and composition of valerian. Journal of herbs, spices & medicinal plants, 14: 54–67.

Ye, F., Liang, Q., Li, H. and Zhao, G. 2015. Solvent effects on phenolic content, composition, and antioxidant activity of extracts from florets of sunflower (Helianthusannuus L.). Industrial Crops and Products, 76: 574–581.

Zargari, A. 1991. Medicinal plants. Tehran: Tehran University Publications, pp. 77-83.