Antidepressant activity of methanol stem bark extract of Ficus platyphylla Del (Moraceae) in mice

Document Type: Original article

Authors

1 Department of Pharmacology and Therapeutics, Ahmadu Bello University, Nigeria

2 Department of Pharmacognosy and Drug Development, Ahmadu Bello University, Nigeria

Abstract

Background Aim: Depression is a heterogeneous mood disorder affecting both people in developing and developed countries. The drugs used in its management are associated with adverse effects and delayed response which compromise their therapeutic benefits. This makes it worthwhile to look for antidepressants plants with proven advantage and favourable benefit-to-risk ratio. Ficusplatyphylla is used traditionally in West Africa for the management of mental illnesses.The aim of this study was to evaluate the antidepressant potential of the methanol stem bark extract of F.platyphylla.
Experimental: Thin layer chromatographic finger prints of the extract was established.  The oral median lethal dose of the extract was estimated using OECD 420 guidelines. Antidepressant activity of the extract at doses of 125-500 mg/kg was evaluated using tail suspension test (TST) and forced swim test (FST). The Effects of the extract on motor co-ordination deficit and locomotion were evaluated using beam walking assay (BWA) and open field test (OFT) respectively.
Results and Discussion: The chromatographic profile suggested the presence of steroids, tannins, flavonoids, alkaloids and saponins. The LD50 was found to be ≥2000 mg/kg orally. The extract significantly (p<0.01) and dose dependently (125, 250 and 500 mg/kg) decrease the duration immobility in the TST and FST. There is no significant increase in the number of lines crossed and on the number of foot slips in the OFT and BWA respectively.
Industrial and Practical recommendations: The methanol stem bark extract of F.platyphylla possesses antidepressant activity. The phytoconstituents found present in the plant assumed to be responsible for the activity can be characterized and isolated to serve as lead compounds in the treatment of depression.

Keywords


Article Title [Persian]

فعالیت ضد افسردگی عصاره متانولی ساقه (Ficus platyphylla Del (Moraceae در موش

Authors [Persian]

  • شیهو آیشاتو 1
  • نبارا اومی 1
  • ماگاجی محمد گاربا 1
  • یو جمیلو 1
  • احمد ابوبکر 2
1 Department of Pharmacology and Therapeutics, Ahmadu Bello University, Nigeria
2 Department of Pharmacognosy and Drug Development, Ahmadu Bello University, Nigeria
Abstract [Persian]

Background Aim: Depression is a heterogeneous mood disorder affecting both people in developing and developed countries. The drugs used in its management are associated with adverse effects and delayed response which compromise their therapeutic benefits. This makes it worthwhile to look for antidepressants plants with proven advantage and favourable benefit-to-risk ratio. Ficusplatyphylla is used traditionally in West Africa for the management of mental illnesses.The aim of this study was to evaluate the antidepressant potential of the methanol stem bark extract of F.platyphylla.
Experimental: Thin layer chromatographic finger prints of the extract was established.  The oral median lethal dose of the extract was estimated using OECD 420 guidelines. Antidepressant activity of the extract at doses of 125-500 mg/kg was evaluated using tail suspension test (TST) and forced swim test (FST). The Effects of the extract on motor co-ordination deficit and locomotion were evaluated using beam walking assay (BWA) and open field test (OFT) respectively.
Results and Discussion: The chromatographic profile suggested the presence of steroids, tannins, flavonoids, alkaloids and saponins. The LD50 was found to be ≥2000 mg/kg orally. The extract significantly (p<0.01) and dose dependently (125, 250 and 500 mg/kg) decrease the duration immobility in the TST and FST. There is no significant increase in the number of lines crossed and on the number of foot slips in the OFT and BWA respectively.
Industrial and Practical recommendations: The methanol stem bark extract of F.platyphylla possesses antidepressant activity. The phytoconstituents found present in the plant assumed to be responsible for the activity can be characterized and isolated to serve as lead compounds in the treatment of depression.

Keywords [Persian]

  • Ficusplatyphylla
  • Antidepressants
  • Depression tail suspension test
  • Forced swim test
Alpermann, H.G., Schaut, U., Usinger, P. and Hock, F.J. 1992. Pharmacological effects of Hoc 249: A new potential antidepressant, Drug Development Research, 25: 267-282.

Amos, S., Chindo, B.A., Edmond, I., Akah, P.,Wambebe, C. and Gamaniel., K. 2008. Anti-inflammatory and antinociceptive effects of Ficusplatyphylla Extract in Mice and Rats.Journal of Herbs, Spices and Medicinal Plants, 9: 47-53.

Antunes, M. and Biala, G. 2012. The novel object recognition memory: neurobiology, test procedure and its modifications. Cognitive Processing, 13: 93-110.

Ashok, K.B.S., Lakshman, K., Velmurugan, C., Sridhar, S.M. and Gopisetty, S. 2013. Antidepresant Activity of Methanolic Extract of Amaranthusspinosus, Basic and Clinical Neuroscience, 5: 11-17.

Bourin, M. 1990. Is it possible to predict the activity of a new antidepressant in animals with simple psychopharmacological tests? Fundamentals ofClinicalPharmacology, vol. 4, pp49–64.

Chindo, B.A., Anuka, J.A., McNeil, L., Yaro, A.H., Adamu, S.A., Amos, A., Connelly, W.K., Lees, G., Gamaniel, K.S. 2009. Anticonvulsant properties of Saponins from Ficusplatyphylla stem bark.Brain Research Bulletin, 786: 276-282.

Chindo, B.A., Yau, J., Danjuma, N.M, Okhale, S.E.,Gamaniel, K.S. and Becker, A. 2014. Behavioural and anticonvulsant effects of the standardized extract of Ficusplatyphylla stem bark. Journal ofEthnopharmacology, 154: 351-60.

Cryan, J.F., Markou, A. and Lucki, I. 2002. Assessing antidepressant activity in rodents: recent development and future need. Trends in Pharmacological Sciences, 23: 238-245.

Cryan, J.F., Mombereau,C.andVassout, A. 2005. The tail suspension test as a model for assessing antidepressant activity: review of pharmacological and genetic studies in mice. Neuroscience and Behavioural Reviews, 29: 571-625.

Detke, M.J., Rickels, M. and Lucki, I. 1995. Assessing antidepressant activity in rodents: recent developments and future need. Trends in Pharmacological Sciences, 121: 66-72.

Dhingra, D. and Sharma, A. 2006. A review on antidepressant plants, Natural Product Radiance; 5: 144-152.

Emamghoreishi, M. and Talebianpour, M.S. 2009. Antidepressant effect of Melissaofficinalis in the forced swim test. DARU Journal of Pharmaceutical Sciences, 17: 42-47.

Leger, M., Quideville, A., Bouet, V., Haelewyn, B., Boulard, M., Schumann-Bard, P. and Freret, T. 2013. Object recognition test in mice. Nature Protocols, 8: 2531-2537.

Mamta, S., Jyoti, S., Rajeev, N., Dharmendra, S. and Abhishek, G. 2013. Phytochemistry of Medicinal Plants. Journal ofPharmacognosy andPhytochemistry, 1: 168-182.

Marston, A. and Hostettmann, K. 1991 Modern separation methods. Natural Product Representative; 8: 391-413.

Nnabuk, O.E., Paul, O.A., Casimir, E.G. and Eno, E.E. 2012. Rheological Modeling and Characterization of Ficusplatyphylla Gum exudates. Journal of Chemistry, 2013: 1-10.

Porsolt, R.D., Anton, G., Blavet, N. and Jalfre, M. 1978. Behavioural despair in rats: a new model sensitive to antidepressive treatments. EuropeanJournal of Pharmacology, 47: 379-391.

Priyanka, B., Sridhar, Y. and Shankaraih, P. 2012. Antidepressant and muscle relaxant activity of Cardios