Firouznia, A., Doustzadeh, F., Rabie, N. (2018). A Comparative Study of Chemical Composition and Antibacterial Properties of Essential Oils of Tanacetum turcomanicum (Krash.) and Tanacetum walteri (C.Winkl.) from Northern Khorasan Province. Journal of Herbal Drugs
(An International Journal on Medicinal Herbs), 9(1), 39-45.
Ali Firouznia; Fahimeh Doustzadeh; Narges Rabie. "A Comparative Study of Chemical Composition and Antibacterial Properties of Essential Oils of Tanacetum turcomanicum (Krash.) and Tanacetum walteri (C.Winkl.) from Northern Khorasan Province". Journal of Herbal Drugs
(An International Journal on Medicinal Herbs), 9, 1, 2018, 39-45.
Firouznia, A., Doustzadeh, F., Rabie, N. (2018). 'A Comparative Study of Chemical Composition and Antibacterial Properties of Essential Oils of Tanacetum turcomanicum (Krash.) and Tanacetum walteri (C.Winkl.) from Northern Khorasan Province', Journal of Herbal Drugs
(An International Journal on Medicinal Herbs), 9(1), pp. 39-45.
Firouznia, A., Doustzadeh, F., Rabie, N. A Comparative Study of Chemical Composition and Antibacterial Properties of Essential Oils of Tanacetum turcomanicum (Krash.) and Tanacetum walteri (C.Winkl.) from Northern Khorasan Province. Journal of Herbal Drugs
(An International Journal on Medicinal Herbs), 2018; 9(1): 39-45.
A Comparative Study of Chemical Composition and Antibacterial Properties of Essential Oils of Tanacetum turcomanicum (Krash.) and Tanacetum walteri (C.Winkl.) from Northern Khorasan Province
Department of Chemistry, Bojnourd Branch, Islamic Azad University, Bojnourd, Iran;
Abstract
Background & Aim: Tanacetum is a member of Asteraceae family and its twelve species such as Tanacetum walteri are endemic to Iran. In this investigation, chemical compounds and antimicrobial activity of essential oils of Tanacetum turcomanicum (Krash.) and Tanacetum walteri (C.Winkl.) were studied. Experimental: The plants were collected from north Khorasan province of Iran, in spring of 2016. The essential oils were extracted by the hydrodistillation method using clevenger. Identification of the essential oils chemical composition was conducted by GC/MS instrument. Also, Agar well diffusion method was used to investigate its antibacterial effects against E. coli, S. aureus, B. atrophaeus and M. luteus. Results: Essential oil efficiency of T. walteri species was obtained 0.2% v/w. In total 92.84 percent of essence constituents of 43 components were recognized in which α-pinene (22.37%), 1,8-cineole (12.52%), camphor (11.91%) and ß-thujone (4.52%) were recognized as the main constituents. By isolating essence of T. turcomanicum species, 39 constituents of 89.28% of the whole essential oils were recognized with efficiency of 0.3% v/w. Davanone (20.79%), 1,8-cineole (15.63%), p-cymene (10.20%), camphor (10.14%) and borneol (4.95%) were the main components of the essence. The highest antimicrobial activity of the essential oils of T. turcomanicum and T. walteri were observed against M. luteus, B. Atrophaeus and S. aureus, respectively. The essential oil of T.turcumanicum did not show significant activity against E. coli bacterium. Recommended applications/industries: The results showed the environmental influences and the effect of plant species on the amount and type of volatile components of essential oils. Also, antibacterial efficacy shown by these oils provides a scientific basis and thus validates their use as medicinal remedies. Isolation and purification of different phytochemicals may yield to significant antibacterial agents.
مطالعه مقایسه ای ترکیبات شیمیایی و خواص ضد باکتریایی روعن های اسانسی مینای ترکمنستانی( Tanacetum turcomanicum (Krash و مینای کپت داغی(Tanacetum walteri (C.Winkl) از استان خراسان شمالی
Authors [Persian]
علی فیروزنیا; فهیمه دوست زاده; نرگس ربیعی
گروه شیمی، دانشگاه آزاداسلامی واحد بجنورد، بجنورد، ایران
Abstract [Persian]
زمینه و هدف: جنس مینا، از تیره کاسنی می باشد که تعداد 12 گونه از آن مانند مینای کپت داغی انحصاری ایران است. در این تحقیق، ترکیبات شیمیایی و فعالیت ضدمیکروبی اسانسهای مینای ترکمنستانی و مینای کپت داغی مورد مطالعه قرار گرفت.
روش تحقیق:اندامهای هوایی گیاهان مورد مطالعه در بهار 1395 از استان خراسان شمالی، ایران جمع آوری شد. اسانسها به روش تقطیر با آب و با استفاده از دستگاه کلونجر استخراج شدند. شناسایی ترکیبات شیمیایی اسانس توسط دستگاه GC/MS انجام شد. همچنین برای بررسی اثرات ضد میکروبی اسانس بر روی باکتریهای اشرشیا کلی، استافیلوکوکوس اورئوس، باسیلوس آتروفئوس و میکروکوکوس لوتئوس از روش انتشار در آگار استفاده شد. نتایج: راندمان اسانس گونه مینای کپت داغی 2/0درصد حجمی/وزنی به دست آمد. در مجموع 84/92 درصد از کل ترکیبات اسانس شامل 43 ترکیب شناسایی گردید. آلفا- پینن (37/22 درصد)، 1و8-سینئول ( 52/12 درصد)، کامفور ( 91/11 درصد) و بتا- توجون (52/4 درصد) به عنوان ترکیبات اصلی شناسایی شدند. از تجزیهی اسانس گونهی مینای ترکمنستانی،39 ترکیب و 28/89 درصد از کل ترکیبات اسانس با راندمان 3/0 درصد حجمی/ وزنی شناسایی شدند. داوانون (79/20 درصد)، 1و8-سینئول (63/15 درصد)، پاراسیمن (20/10 درصد)، کامفور (14/10 درصد) و بورنئول (95/6 درصد) ترکیبات اصلی اسانس را تشکیل می دادند. بالاترین فعالیت ضد میکروبی برای اسانسهای مینای ترکمنستانی و مینای کپت داغی به ترتیب در برابر باکتریهای میکروکوکوس لوتئوس، باسیلوس آتروفئوس و استافیلوکوکوس اورئوس مشاهده شد. اسانس مینای ترکمنستانی، هیچ اثر معنا داری را در برابر باکتری اشرشیا کلی نشان نداد. توصیه های کاربردی و صنعتی:نتایج نشان میدهد که تاثیرات محیطی و نوع گونه های گیاهی بر مقدار و نوع اجزای فرار اسانسها تأثیر میگذارد. همچنین، اثر ضد باکتریایی نشان داده شده توسط این اسانسها پایه ی علمی را فراهم کرده که استفاده از آنها در درمانهای دارویی معتبر می باشد. جداسازی و خالص سازی ترکیبات گیاهی مختلف، ممکن است ترکیباتی با خاصیت ضد میکروبی بیشتر را فراهم آورد.
Keywords [Persian]
ضد میکروبی، اسانس، GC/MS، مینای ترکمنستانی، مینای کپت داغی
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