Plant growth promoting rhizobacteria enhance oil content and physiological status of Thymus daenensis Celak. under drought stress

Document Type: Original article

Authors

1 Soil Sciences department, Agriculture Faculty, Lorestan University, Khurramabad, Iran

2 Soil Sciences department, Agriculture Faculty, Lorestan University, Khorramabad, Iran.

3 Soil Sciences department, Agriculture Faculty, Isfahan University of Technology, Isfahan, Iran.

Abstract

Background & Aim:Currently, the use of beneficial microorganisms with the hope to reduce the adverse effects of drought has been the focus of attention. In present study, the effects of plant growth promoting rhizobacteria (PGPR) and drought stress on essential oil (EO) and physiological status of Thymus daenensis Celak, were investigated.
Experimental: The experiment was arranged as a factorial pattern in a randomized complete design with three replications. Factors were four irrigation regimes included: well-watered (A: absence of stress), irrigation after depletion of 20-25% of field capacity (L: low stress), irrigation after depletion of the 35-40% of field capacity (M: mild stress) and irrigation after depletion of the 55-60% of field capacity (S: severe stress). Also, two PGPR treatments, non-inoculation (C: control) and inoculation with PGPR were conducted.
Results:The results showed that drought stress reduced root and shoot dry weight, relative water content, photosynthetic pigments and gas change parameters but PGPR inoculation improved all of them. Proline, malondialdehyde, electrolyte leakage and stomatal resistance increased with increasing water stress, but PGPR inoculation ameliorate these increases in corresponding treatments. PGPR inoculation increased essential oil production although this increase was not statistically significant but water stress decreased it.
Recommended applications/industries:The results suggest that PGPR inoculation could be an excellent strategy to alleviate adverse effects of water stress in Thymus daenensis cultivation in drought stress conditions. Therefore, farmers in semiarid regions could produce T. daenensis by using of PGPR at low water stress for the highest economic amount of extracted essential oil.

Keywords


Article Title [Persian]

تاثیر باکتریهای افزایش دهنده رشد گیاه بر عصاره و فاکتورهای فیزیولوژیکی آویشن دنایی (Thymus daenensis Celak) در شرایط تنش خشکی

Authors [Persian]

  • علی عبداللهی 1
  • محمد فیضیان 2
  • غزاله مهدی پوریان 3
1 گروه خاکشناسی ، دانشکده کشاورزی ، دانشگاه لرستان ، خرم آباد ، ایران؛
2 گروه خاکشناسی، دانشکده کشاورزی ، دانشگاه لرستان ، خرم آباد ، ایران؛
3 گروه خاکشناسی ، دانشکده کشاورزی ، دانشگاه صنعتی اصفهان ، اصفهان ، ایران؛
Abstract [Persian]

زمینه و هدف: در حال حاضر استفاده از میکروارگانیسم های مفید به عنوان نهاده های کشاورزی پایدار و کارآمد با امیدواری در کاهش اثرات منفی خشکسالی مورد توجه قرار گرفته است. به منظور بررسی تأثیر ریزوباکتری های تقویت کننده رشد گیاه (PGPR) و تنش خشکی بر اسانس (EO) و فاکتورهای فیزیولوژیکی آویشن دنایی (Thymus daenensis Celak)، یک آزمایش در سال 2017 در شهرکرد انجام شد.
شرح آزمایش: آزمایش به صورت فاکتوریل در قالب طرح کاملا تصادفی با سه تکرار انجام شد. فاکتورها چهار رژیم آبیاری و دو تلقیح PGPR بودند. فاکتور اول شامل چهار رژیم آبیاری عبارت بودند از: آبیاری کامل (A: عدم استرس) ، آبیاری پس از کاهش 20-25٪ ظرفیت مزرعه (FC) (L: استرس کم) ، آبیاری پس از کاهش 35-40 FC (M: استرس خفیف) و آبیاری پس از کاهش 60-55٪ FC (S: استرس شدید). فاکتور دوم شامل دو تیمار PGPR ، عدم تلقیح (C: شاهد) و تلقیح با PGPR (M: Pseudomonas fluorescens سویه 187 و Pseudomonas aeruginosa سویه MPFM) بود.
یافته ها: نتایج نشان داد که تنش خشکی باعث کاهش وزن خشک ریشه و شاخساره ، محتوای نسبی آب ، رنگدانه های فتوسنتزی و پارامترهای تبادل گازی شد اما تلقیح PGPR باعث بهبود آنها گردید. با افزایش تنش خشکی ، پرولین ، مالون دی آلدئید ، نشت الکترولیت و مقاومت روزنه ای افزایش یافت، اما تلقیح PGPR باعث کاهشاین فاکتورها در تیمارهای مربوطه شد. تلقیح PGPR باعث افزایش تولید اسانس گردید اگرچه این افزایش از نظر آماری معنی دار نبود اما تنش آب تولید اسانس را کاهش داد.
توصیه های صنعتی و عملی: نتایج نشان می دهد که تلقیح PGPR می تواند یک استراتژی عالی برای کاهش اثرات منفی تنش خشکی در کشت آویشن دنایی در شرایط تنش خشکی باشد، بنابراین کشاورزان مناطق نیمه خشک می توانند با استفاده از PGPR در تنش کم آب بیشترین مقدار EO در در آویشن دنایی تولید کنند.

Keywords [Persian]

  • آویشن دنایی (Thymus daenensis Celak)
  • اسانس
  • باکتریهای افزایش دهنده رشد گیاه
  • تنش خشکی
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