In vitro investigation of antibacterial properties of Citrus medica essential oil against some human pathogenic bacteria
Subject Areas : Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)
محدثه
شجاعی مهر
1
(گروه بیوتکنولوژی گیاهی، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران)
مصطفی
علم هولو
2
(گروه بیوتکنولوژی، دانشکده کشاورزی، دانشگاه بوعلی سینا همدان)
Keywords: Antibacterial, Essential oil, Citrus medida, anti- radical,
Abstract :
Background & Aim: The medicinal plants are used in treatment of diseases caused by the human pathogenic bacteria due to their antimicrobial compounds. The aim of this study was to investigate antibacterial and antioxidant activity of Citrus medica essential oil on some human pathogenic bacteria. Experimental: The plant samples of Citrus medica were collected from North of Iran. Samples were transferred to the biotechnology laboratory, Bu Ali Sina University, Hamadan. The essential oil was extracted by Clevenger device. Antibacterial activity and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by agar well diffusion and by micro dilution broth methods, respectively. Antiradical activity was evaluated by 2,2-diphenyl-1-picrylhydrazyl assay (DPPH). Results: The highest and lowest inhibitory activity of essential oil was observed on Bacillus cereus and Escherichia coli, respectively. Leaf essential oil showed the highest IC50 value than the skin essential oil. The essential oil of Citrus medica skin showed more inhibitory effect than the leaf essential oil. The MIC of leaf essential oil on B. subtilis, B. cereus and E. aerogenes and the MBC on B. cereus were found to be 3.12 mg ml-1. The MIC of skin essential oil on M. luteus was 1.56 mg ml-1 and the MBC on M. luteus and S. aureus was 3.12 mg ml-1. Recommended applications/industries: The results confirmed the efficacy of C. medica essential oil as natural antimicrobial and suggested the possibility of employing it in drugs for the treatment of diseases caused by the test organisms.
Alamhulu, M. and Nazeri, S. 2015a. Investigation antibacterial and antioxidant activities of alcoholic extracts of flower and root Dendro stelleralesserti on some human pathogenic bacteria. Scientific Journal of Hamadan University of Medical Sciences, 21(4): 277-285.
Alamhulu, M. and Nazeri, S. 2015b. Assessment of the antioxidant and antibacterial effects of stem and leaf alcoholic extracts of Dendrostelleralesserti. Journal of Microbial World, 7(4): 289-298.
Alamhulu, M. and Nazeri, S. 2016. The in vitro antibacterial activity of different organs hydroalcoholic extract of Dendro stelleralesserti. Journal of Plant Researches, 29 (3): 534-542.
Ayoola, G.A., Johnson, O.O., Adelowotan,T., Aibin, I.E., Adenipekun, E. and Odugbemi, T.O. 2008. Evaluation of the chemical constituents and theantimicrobial activity of the volatile oil of Citrus reticulata fruit (Tangerine fruit peel) from South West Nigeria. African Journal of Biotechnology, 7 (13): 2227-2231.
Badar, N., Arshad, M. and Farooq, U. 2008. Characteristics of Anethum graveolens (Umbelliferae) seed oil: Extraction, composition and antimicrobial activity. International Journal of Agriculture and Biology, 10: 329-332.
Caccioni, D.R., Guizzardi, M., Biondi, D.M., Renda, A. and Ruberto, G. 1998. Relationship between volatile components of Citrus fruit essential oils and antimicrobial action on Penicillium digitatum and Penicillium italicum. International Journal of Food Microbiology, 43: 73–79.
Choi, H.S., Song, H.S., Ukeda, H. and Sawamura, M. 2000. Radical-scavenging activities of Citrus essential oils and their components: Detection using 1, 1-diphenyl-2-picrylhydrazyl. Journal of Agriculture and Food Chemistry, 48: 4156–4161.
Delaquis, P.J., Stanich, K., Girard, B. and Mazza, G. 2002. Antimicrobial activity of individual and mixedfractions of dill, cilantro, coriander and eucalyptusessential oils. International Journal of Food Microbiology, 74: 101-109.
Geyas, F., Young, E., Blankenship, S.M. and McFeeters, R.F. 1996. Dietary fiber composition of stark spur supreme delicious applefruit as influenced by rootstock and growing region. Fruit Varieties Journal, 1: 35–41.
Gonzalez, C.N., Sanchez, F. and Quintero, A. 2002. Chemotaxonomic value of essential oil compounds in Citrus species. Acta Horticulturae, 576: 49-51.
Hamendra, S.P. and Anand, K. 2007. Antidiabetic potential of Citrus sinensis and Punica granatum peel extracts in alloxan treated male mice. BioFactors, 31:17–24
Han, S.T. 1998. Medicinal plants in the south pacific. World Health Organization, Regional Publications West Pacific Series 19, Manila.
Jafari, S., Esfahani, S., Fazeli, M.R., jamalifar, H., Ardekani, M.R. and Khanavi, M. 2011. Antimicrobial activity of lime oil agaisnt food-borne pathogens isolated from cream-filled cakes and pastries. International Journal of Biological and Chemical Sciences, 5(4): 226-258.
Kamal, GM., Ashraf, M.Y., Hossein, A., Shahzadi, A. and Ghughtai, I.C. 2013. Amtioxidant potential of peel essential oil of three Pakistani Citrus species: Citrus reticulate, Citrus sinensisand Citrus paradise. Pakistan Journal of Botany, 45(4): 1449-1454.
Menichini, F., Tundis, R., Bonesi, M., Cindio, B., Loizzo, M. R., Conforti, F., Statti, G.A., Menabeni, R., Bettini, R. and Menichini, F. 2011. Chemical composition and bioactivity of Citrus medica L. cv. Diamante essential oil obtained by hydrodistillation, cold-pressing and supercritical carbon dioxide extraction. Natural product research, 25(8):789-799.
Mokbel, M.S. and Suganuma, T. 2006. Antioxidant and antimicrobial activities of the methanol extracts from pummelo (Citrus grandis) fruit albedo tissues. European Food Research and Technology, 224(1): 39-47.
Odugbemi, T.O. 2006. Outlines and pictures of medicinal plants from Nigeria. University of Lagos Press, Lagos, Nigeria.
Oh, H.J., Ahn, H.M., Kim, S.S., Yun, P.Y. and Riu, KZ. 2007. Composition and antimicrobial activities of essential oils in the peel of Citrus Fruits. Applied Biological Chemistry, 50(3): 148-154.
Okunowo, W.O., Oyedeji, O., Afolabi, L.O. and Matanmi, E. 2013. Essential oil of grape fruit (Citrus paradisi) peels and its antimicrobial activities. American Journal of Plant Sciences, 4: 1-9.
Ramadan, W., Mourad, B., Ibrahim, S. and Sonbol, F. 1996. Oil of bitter orange: new topical antifungal agent. International Journal of Dermatology, 35(6): 448-9.
Sahin, F., Gulluce, M., Daferera, D., Sokmen, A., Sokmen, M. and Polissiou, M. 2004. Biological activities of the essential oils and methanol extract of Origanum vulgare ssp. vulgare in the eastern anatolia region of Turkey. Food Control, 15(7): 549-557.
Sarrou, E., Chatzopoulou, P., Theriou, D.K. and Therios, I. 2013. Volatile constituents and antioxidant activity of peel, flowers and leaf pils of Citrus aurantium L. Growing in Greece. Molecules, 18:10639-10647.
Sharma, N. and Tripathi, A. 2008. Effects of Citrus sinensis (L.) Osbeck epicarp essential oil on growth and morphogenesis of Aspergillus niger (L.) Van Tieghem. Microbiology Research, 163(3):337-44.
Shojaemehr, M. and Alamholo, M. 2019. Antibacterial activity of alcoholic and aqueous extracts of various organs of Citrus medica on 10 human pathogenic in vitro. Iranian Journal of Medical Microbiology, 13 (4): 310-320.
Sokovic, M., Marin, P.D., Brkic, D. and van Griensven, L.J.L.D. 2007. Chemical composition and antibacterial activity ofessentialoils of ten aromatic plants against human pathogenic bacteria.Global Science Books.
Srisukh, V., Tribuddharat, C.H., Nukoolkarn, V., Bunyapraphatsara, N., Chokephaibulkit, K. and Srifuengfung, S. 2012. Antibacterial activity of essential oils from Citrus hystrix (makrut lime) against respiratory tract pathogens. Science Asia, 38: 212–217.
Stange, J.R.R.R., Midland, S.L., Eckert, J.W. and Sims, J.J. 1993. An Antifungal compound produced by grapefruit and valencia orange after wounding of the wounding of the peel. Journal of Natural Products, 56:1637–1654.
Stojicevic, S.S., Stanisiavljevic, I.T., Velickovic, D.T., Veljkovic, V.B. and Lazic M.L. 2008. Comparative screening of the anti-oxidant and antimicrobial activities of Sempervivum marmoreum L. extracts obtained by various extraction techniques. Journal of the Serbian Chemical Society, 73(6): 597-60.
Tao, N.G. and Liu,Y.J. 2012. Chemical composition and antimicrobial activity of the essential oil from the peel of Shatian pummelo. International Journal of Food Properties, 15: 709-716.
Theanphong, O., Songsak, T. and Mingvanish,W. 2008. Chemical composition and antimicrobial activity of the essential oil from Citrus medica L. var. sarcodactylis (Sieber) Swingle leaf. Mahidol University Journal of Pharmaceutical Sciences, 35(1-4): 57-61.
Upadhyay, R.K., Dwivedi, P. and Ahmad, S.h.2010. Screening of antibacterial activity of six plant essential oilsagainst pathogenic bacterial strain. Asian Journal of Medical Sciences, 2(3): 152-158.
Walsh, S.E.J.Y., Maillard, A.D., Russel, C.E. and Catrenich, D.L. 2003. Activityand mechanism of action of selected biocidalagentson Gram - positive and negative bacteria. Journal of Applied Microbiology, 94: 240-247.