Ameliorative properties of methanol leaf extract of Momordica charantia following alloxan-induced hepatotoxicity in rats

Document Type: Original article

Authors

1 Department of Veterinary Pharmacology and Toxicology University of Ibadan, Nigeria

2 Department of Veterinary Physiology and Biochemistry University of Ibadan Ibadan, Nigeria

3 Department of Veterinary Medicine University of Ibadan Ibadan, Nigeria

4 Department of Pharmacology and Therapeutics University of Ibadan Ibadan, Nigeria

5 Department of Botany University of Ibadan Ibadan, Nigeria

6 Department of Environmental & Interdisciplinary Sciences, College of Science, Engineering & Technology, Texas Southern University, Houston, TX, USA

7 Department of Biomedical Sciences Cape Peninsula University of Technology Bellsville, South Africa.

8 Department of Veterinary Pharmacology and Toxicology Faculty of Veterinary Medicine University of Ibadan

Abstract

Background & Aim:Momordica charantiais an extensively distributed plant that is broadly prescribed in African medical system for treatment of various ailments. The plant has a comprehensive range of therapeutic uses. The present study reveals the antioxidative and cardioprotective abilities of methanol leaf extract of Momordica charantia (MLEMC) against cardiotoxicity using alloxan-induced animal model.
Experimental: The ameliorative effect of the methanol leaf extract of Momordica charantia (MLEMC) was studied in alloxan-induced cardiac injury in 50 rats divided into five groups (A-E) (n =10) i.e. group A control, group B was toxicant group, group C animals received glibenclamide treatment while groups D and E received extracts at 200 and 400 mg/kg doses, respectively, for 28 days. Histopathological changes, serum cardiac injury markers such as myeloperoxidase (MPO) activity, nitric oxide (NO) contents; oxidative status, blood pressure, electrocardiogram, cardiac P38 and CRP were evaluated.
Results: The extract-treated group showed a decreased level of oxidant markers such as malondialdehyde(MDA) and hydrogen peroxide(H2O2) but increased levels of protein thiols, non-protein thiols, glutathione (GSH), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and superoxide dismutase (SOD) indicating its anti-oxidant potential. Heart sections revealed mild distortion of the cardiac architecture compared to toxicant group while decreased expression of cardiac P38 and CRP in extract-treated groups was observed.
Recommended applications/industries: The plant extract exhibited anti-oxidant and anti-inflammatory effects, thereby displaying cardio-protective property which propose the plant as a good natural source for herbal nutraceuticals.

Keywords


Article Title [Persian]

خواص تقویتی عصاره متانولی برگ Momordica charantia در سمیت های کبدی ناشی از آلوکسان در موش صحرایی

Authors [Persian]

  • ساندی افوب 1
  • آدمولا اویابمی 2
  • تمیدایو اومواله 3
  • ادورابنرو آدداپو 4
  • آبیدون آیودل 5
  • مومح یاکوبو 6
  • الوفمی اگانتیبجو 7
  • آدلو آدداپو 8
1 گروه فارماکولوژی و سم شناسی دانشکده دامپزشکی، دانشگاه ابادان ، نیجریه
2 گروه فیزیولوژی و بیوشیمی دانشکده دامپزشکی، دانشگاه ابادان ، نیجریه
3 گروه دامپزشکی دانشگاه ابادان ابادان ، نیجریه
4 گروه فارماکولوژی، دانشگاه علوم پزشکی ایبادان ابادان ، نیجریه
5 گروه گیاهشناسی دانشگاه ابادانان ابادان ، نیجریه
6 گروه محیط زیست ،دانشکده علوم ، مهندسی و آمپر؛ فناوری ، دانشگاه جنوبی تگزاس ، هیوستون ، ایالات متحده
7 گروه علوم زیست پزشکی دانشگاه صنعتی شبه جزیره کیپ بلزویل ، آفریقای جنوبی.
8 گروه فارماکولوژی دامپزشکی و سم شناسی دانشکده دامپزشکی دانشگاه ابادان
Abstract [Persian]

Background & Aim:Momordica charantiais an extensively distributed plant that is broadly prescribed in African medical system for treatment of various ailments. The plant has a comprehensive range of therapeutic uses. The present study reveals the antioxidative and cardioprotective abilities of methanol leaf extract of Momordica charantia (MLEMC) against cardiotoxicity using alloxan-induced animal model.
Experimental: The ameliorative effect of the methanol leaf extract of Momordica charantia (MLEMC) was studied in alloxan-induced cardiac injury in 50 rats divided into five groups (A-E) (n =10) i.e. group A control, group B was toxicant group, group C animals received glibenclamide treatment while groups D and E received extracts at 200 and 400 mg/kg doses, respectively, for 28 days. Histopathological changes, serum cardiac injury markers such as myeloperoxidase (MPO) activity, nitric oxide (NO) contents; oxidative status, blood pressure, electrocardiogram, cardiac P38 and CRP were evaluated.
Results: The extract-treated group showed a decreased level of oxidant markers such as malondialdehyde(MDA) and hydrogen peroxide(H2O2) but increased levels of protein thiols, non-protein thiols, glutathione (GSH), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and superoxide dismutase (SOD) indicating its anti-oxidant potential. Heart sections revealed mild distortion of the cardiac architecture compared to toxicant group while decreased expression of cardiac P38 and CRP in extract-treated groups was observed.
Recommended applications/industries: The plant extract exhibited anti-oxidant and anti-inflammatory effects, thereby displaying cardio-protective property which propose the plant as a good natural source for herbal nutraceuticals.

Keywords [Persian]

  • Momordica charantia
  • Cardiotoxicity
  • Diabetes
  • Oxidative stress
  • Immunohistochemistry
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