Antidiabetic and anti-oxidant effects of methanol leaf extract of Momordica charantia following alloxan-induced hyperglycaemia in rats

Document Type: Original article

Authors

1 Department of Veterinary Pharmacology and Toxicology, University of Ibadan, Nigeria;

2 Department of Veterinary Medicine, University of Ibadan, Nigeria;

3 Department of Veterinary Physiology and Biochemistry, University of Ibadan, Nigeria;

4 Department of Environmental and Interdisciplinary Sciences, COSET, Texas Southern University, Houston, TX, USA;

5 Department of Biomedical Sciences, Cape Peninsula University of Technology, Bellsville, South Africa;

6 Department of Veterinary Pharmacology and Toxicology Faculty of Veterinary Medicine University of Ibadan

Abstract

Background & Aim:The plant Momordica charantia is highly valuable having a wide range of medicinal uses with tropical and subtropical regions distribution. This study was conducted to appraise the medicative properties of methanol leaf extract of Momordica charantia (MEMC) on alloxan-induced diabetic rats.
Experimental: The antidiabetic influence of methanol leaf extract of Momordica charantia (MEMC) was investigated in alloxan-induced diabetes in rats. 50 rats allocated into five groups (A-E) (n =10) were utilized in this study: group A was normal control, groups B to E were induced with alloxan with diabetes established, while group B was not treated, group C received glibenclamide treatment while groups D and E were administered extracts at 200 and 400 mg/kg doses, respectively, in a study that continued for 28 days. Changes in blood glucose levels, body weight, haematology, serum chemistry, antioxidant system and histopathology changes were evaluated.
Results: MEMC elicited significant drop in blood glucose level from diabetic to near normal level, restoring the body weight, haematological and serum biochemical parameters to the basal non diabetic level; likewise the MEMC-treated group elicited a decreased level of oxidant markers such as malondialdehyde (MDA) and hydrogen peroxide (H2O2) but increased concentration of protein thiols (PT), non-protein thiols (NPT), glutathione (GSH), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and superoxide dismutase (SOD) indicating its anti-oxidant potential. The pancreas section also revealed repair of distorted pancreatic architecture in MEMC-treated group compared to diabetic group.
Recommended applications/industries: The plant exhibited anti-hyperglycaemic, anti-diabetic and anti-oxidant abilities hence could be explored for the treatment of diabetes mellitus.

Keywords


Article Title [Persian]

اثرات آنتی بیوتیکی و آنتی اکسیدانی عصاره متانولی برگ گیاه Momordica charantia بر فشار خون ناشی از آلوکسان در موش صحرایی

Authors [Persian]

  • ساندی افوگ 1
  • الوفکه فلایی 1
  • بلسین اگونپلو 2
  • آدمولا اویابمی 3
  • تمیدایو اوموبوال 2
  • مومو یکوبو 4
  • اولووافمی اوگونتیبجو 5
  • آدلو آدداپو 6
1 گروه فارماکولوژی و سم شناسی دامپزشکی ، دانشگاه ابادان ، نیجریه؛
2 گروه دامپزشکی ، دانشگاه ابادان ، نیجریه؛
3 گروه فیزیولوژی دامپزشکی و بیوشیمی ، دانشگاه ابادان ، نیجریه؛
4 گروه علوم محیط زیست و علوم بین رشته ای ، COSET ، دانشگاه جنوبی تگزاس ، هیوستون ، TX ، ایالات متحده ؛
5 گروه علوم زیست پزشکی ، دانشگاه صنعتی شبه جزیره کیپ ، بلزویل ، آفریقای جنوبی؛
6 گروه فارماکولوژی و سم شناسی دامپزشکی ، دانشگاه ابادان ، نیجریه؛
Abstract [Persian]

Background & Aim:The plant Momordica charantia is highly valuable having a wide range of medicinal uses with tropical and subtropical regions distribution. This study was conducted to appraise the medicative properties of methanol leaf extract of Momordica charantia (MEMC) on alloxan-induced diabetic rats.
Experimental: The antidiabetic influence of methanol leaf extract of Momordica charantia (MEMC) was investigated in alloxan-induced diabetes in rats. 50 rats allocated into five groups (A-E) (n =10) were utilized in this study: group A was normal control, groups B to E were induced with alloxan with diabetes established, while group B was not treated, group C received glibenclamide treatment while groups D and E were administered extracts at 200 and 400 mg/kg doses, respectively, in a study that continued for 28 days. Changes in blood glucose levels, body weight, haematology, serum chemistry, antioxidant system and histopathology changes were evaluated.
Results: MEMC elicited significant drop in blood glucose level from diabetic to near normal level, restoring the body weight, haematological and serum biochemical parameters to the basal non diabetic level; likewise the MEMC-treated group elicited a decreased level of oxidant markers such as malondialdehyde (MDA) and hydrogen peroxide (H2O2) but increased concentration of protein thiols (PT), non-protein thiols (NPT), glutathione (GSH), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and superoxide dismutase (SOD) indicating its anti-oxidant potential. The pancreas section also revealed repair of distorted pancreatic architecture in MEMC-treated group compared to diabetic group.
Recommended applications/industries: The plant exhibited anti-hyperglycaemic, anti-diabetic and anti-oxidant abilities hence could be explored for the treatment of diabetes mellitus.

Keywords [Persian]

  • Diabetes mellitus
  • Momordica charantia
  • Alloxan
  • hydrogen peroxide
  • Malondialdehyde
  • superoxide dismutase
  • Glutathione peroxidase
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