In-vitro antioxidant capacity and hepato-protective potential of Blighia sapida stem bark ether fractions in STZ induced diabetes rats

Document Type: Original article

Authors

1 Department of Biochemistry, Faculty of Science, Adekunle Ajasin University, Akungba Akoko, Nigeria

2 Department of Chemical Sciences, Olusegun Agagu University, Okitipupa, Ondo State, Nigeria

3 Department of Chemical Sciences, Olusegun Agagu University of Science and Technology, Okitipupa, Ondo State, Nigeria

4 Depaetment of Biochemistry, Faculty of Science, Adekunle ajasin University, Akungba-Akoko, Ondo State, Nigeria

Abstract

Background & Aim:Diabetes mellitus is a metabolic disease that affects all systems in the body, including the liver. This study evaluated the in-vitro antioxidant capacity and liver function status of STZ-induced diabetic rats treated with petroleum and diethyl ether fractions of Blighia sapida stem barkfor 14 days.
Experimental: The antioxidant ability of the petroleum and diethyl ether fraction of Blighia sapida stem bark was evaluated by total flavonoids and phenolic content (TFC and TPC) and DPPH scavenging activity using standard protocol. Thirty-five rats in seven groups were used. Plasma transaminases (ALT and AST) activities and bilirubin level was determined using standard procedure.
Results: The TFC and TPC of petroleum ether fraction of B. sapida (PEFBS) (47.16 mg QUE/100g and 39.87 mg GAE/100g) was observed to be higher compared to diethyl ether fraction of B. sapida (DEFBS) (37.44 mg QUE/100 g and 36.74 mg/GAE/100g). The DPPH scavenging activity of the fractions were significantly (P < 0.05) reduced across the concentrations compared to the standard (gallic acid). STZ induced diabetes rats administered 2 ml/kg b. w. of normal saline significantly (p < 0.05) increased plasma ALT, AST activities and bilirubin level compared to the normal control rats while treatment of diabetic rats with petroleum and diethyl ether fraction of B. sapida at both dosesreduced the activities of these enzymes and level of bilirubin.
Recommended applications/industries: The results sustain the fact that, the fractions of B. sapida have an immense potential to be developed further into a therapeutic agent.

Keywords


Article Title [Persian]

ظرفیت آنتی اکسیدانی و پتانسیل محافظت کبدی عصاره اتری پوست ساقه Blighia sapida در موشهای دیابتی ناشی از STZ

Authors [Persian]

  • دامیلولا اوموبوا 1
  • امانوئل آکینتیمین 2
  • سلمنت آکینوبی 3
  • توهیب بالگون 4
1 گروه بیوشیمی ، دانشکده علوم ، دانشگاه Adekunle Ajasin ، Akungba Akoko ، نیجریه
2 گروه علوم شیمی ، دانشگاه اولوسگون آگاگو ، اوکیتیپوپا ، ایالت اوندو ، نیجریه
3 گروه علوم شیمی ، دانشگاه اولوسگون آگاگو ، اوکیتیپوپا ، ایالت اوندو ، نیجریه
4 گروه بیوشیمی ، دانشکده علوم ، دانشگاه Adekunle Ajasin ، Akungba Akoko ، نیجریه
Abstract [Persian]

Background & Aim:Diabetes mellitus is a metabolic disease that affects all systems in the body, including the liver. This study evaluated the in-vitro antioxidant capacity and liver function status of STZ-induced diabetic rats treated with petroleum and diethyl ether fractions of Blighia sapida stem barkfor 14 days.
Experimental: The antioxidant ability of the petroleum and diethyl ether fraction of Blighia sapida stem bark was evaluated by total flavonoids and phenolic content (TFC and TPC) and DPPH scavenging activity using standard protocol. Thirty-five rats in seven groups were used. Plasma transaminases (ALT and AST) activities and bilirubin level was determined using standard procedure.
Results: The TFC and TPC of petroleum ether fraction of B. sapida (PEFBS) (47.16 mg QUE/100g and 39.87 mg GAE/100g) was observed to be higher compared to diethyl ether fraction of B. sapida (DEFBS) (37.44 mg QUE/100 g and 36.74 mg/GAE/100g). The DPPH scavenging activity of the fractions were significantly (P < 0.05) reduced across the concentrations compared to the standard (gallic acid). STZ induced diabetes rats administered 2 ml/kg b. w. of normal saline significantly (p < 0.05) increased plasma ALT, AST activities and bilirubin level compared to the normal control rats while treatment of diabetic rats with petroleum and diethyl ether fraction of B. sapida at both dosesreduced the activities of these enzymes and level of bilirubin.
Recommended applications/industries: The results sustain the fact that, the fractions of B. sapida have an immense potential to be developed further into a therapeutic agent.

Keywords [Persian]

  • Diabetes
  • Antioxidant
  • Steptozotocin
  • liver
  • Transaminases
  • Ether
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