Selection of appropriate HLB values for a stable honey-shea butter emulsion and its efficacy in treating chemical burn wound.

Document Type : Original article

Authors

1 Department of Pharmaceutical Technology and Raw Materials Development, NIPRD, Plot 942, Cadastral Zone C16, Nigeria

2 Department of Pharmacology and Toxicology, NIPRD, Abuja, Nigeria

10.30495/medherb.2021.686191

Abstract

Background & Aim: Since ancient times till today, honey and shea butter are used in wound treatment. This present study aims to determine an effective hydrophile-lipophile balance (HLB) value that will produce a stable W/O honey-shea butter emulsion and to evaluate its healing potential on chemical burn wound in mice.
Experimental: Using various ratios of span 20/tween 20, 6 formulations of honey-shea butter emulsion were prepared and subjected to accelerated stability study as per ICH guideline. The most stable preparation was further evaluated for its wound healing ability. The animals were sacrificed via chloroform inhalation after treatment for 7 days from wound created by dropping 0.2 ml, hydrochloric acid for 15 sec. The burn area was harvested and subjected to histopathological study.
Results: Most stable emulsion was those prepare with ratio 5:5 of span 20/ tween 20. Haematoxylin-eosin and Verhoeff-Van Gieson stain revealed that groups treated with this emulsion had normal fibroblast, fibrocyte, epidermal cells, inflammatory cells, gland cells, collagen and elastic fibres; outperforming honey, shea butter and silver sulfadiazine groups. The present formulation gave a percent wound contraction of 36, while those of honey, shea butter, silver sulfadiazine were 29, 25 and 28, respectively. The occlusive effect of emulsion and the synergistic effect achieved by combining honey and shea butter could also be responsible for its superior wound healing activity.
Recommended applications/industries: Our study concluded that honey-shea butter prepared with 5:5 span 20/tween 20 was relatively stable and possesses greater wound healing activity compared to commercial preparation and can safely be utilize as an effective natural therapy for burn wound.

Keywords


Article Title [فارسی]

انتخاب مقادیر مناسب HLB برای امولسیون پایدار عسل-شی باتر و اثربخشی آن در درمان زخم سوختگی شیمیایی

Authors [فارسی]

  • جانسون ایساک 1
  • عایشا دابوری 2
  • کنچوکوو بن- اومه 1
  • بنت ایفانی 1
  • فیلیپ بیلدرز 1
1 گروه توسعه فناوری دارویی و مواد خام، NIPRD، قطعه 942، منطقه کاداستر C16، نیجریه
2 گروه فارماکولوژی و سم شناسی، NIPRD، ابوجا، نیجریه
Abstract [فارسی]

Background & Aim: Since ancient times till today, honey and shea butter are used in wound treatment. This present study aims to determine an effective hydrophile-lipophile balance (HLB) value that will produce a stable W/O honey-shea butter emulsion and to evaluate its healing potential on chemical burn wound in mice.
Experimental: Using various ratios of span 20/tween 20, 6 formulations of honey-shea butter emulsion were prepared and subjected to accelerated stability study as per ICH guideline. The most stable preparation was further evaluated for its wound healing ability. The animals were sacrificed via chloroform inhalation after treatment for 7 days from wound created by dropping 0.2 ml, hydrochloric acid for 15 sec. The burn area was harvested and subjected to histopathological study.
Results: Most stable emulsion was those prepare with ratio 5:5 of span 20/ tween 20. Haematoxylin-eosin and Verhoeff-Van Gieson stain revealed that groups treated with this emulsion had normal fibroblast, fibrocyte, epidermal cells, inflammatory cells, gland cells, collagen and elastic fibres; outperforming honey, shea butter and silver sulfadiazine groups. The present formulation gave a percent wound contraction of 36, while those of honey, shea butter, silver sulfadiazine were 29, 25 and 28, respectively. The occlusive effect of emulsion and the synergistic effect achieved by combining honey and shea butter could also be responsible for its superior wound healing activity.
Recommended applications/industries: Our study concluded that honey-shea butter prepared with 5:5 span 20/tween 20 was relatively stable and possesses greater wound healing activity compared to commercial preparation and can safely be utilize as an effective natural therapy for burn wound.

Keywords [فارسی]

  • Emulsion
  • Hydrophile-lipophile balance
  • Accelerated stability
  • Wound
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